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Of particular concern is their interaction with adipose tissue, a vital component of the endocrine system regulating metabolic and hormonal functions. The SGBS (Simpson Golabi Behmel Syndrome) cell line, a well\u2010established human\u2010relevant model for adipocyte research, closely mimics native adipocytes' properties. It responds to hormonal stimuli, undergoes adipogenesis and has been successfully used to study the impact of EDCs on adipose biology. In this study, we screened human exposure\u2010relevant doses of various EDCs on the SGBS cell line to investigate their effects on viability, lipid accumulation and adipogenesis\u2010related protein expression. Submicromolar doses were generally well tolerated; however, at higher doses, EDCs compromised cell viability, with cadmium chloride (CdCl<jats:sub>2<\/jats:sub>) showing the most pronounced effects. Intracellular lipid levels remained unaffected by EDCs, except for tributyltin (TBT), used as a positive control, which induced a significant increase. Analysis of adipogenesis\u2010related protein expression revealed several effects, including downregulation of fatty acid\u2010binding protein 4 (FABP4) by dibutyl phthalate, upregulation by CdCl<jats:sub>2<\/jats:sub> and downregulation of perilipin 1 and FABP4 by perfluorooctanoic acid. Additionally, TBT induced dose\u2010dependent upregulation of C\/EBP\u03b1, perilipin 1 and FABP4 protein expression. These findings underscore the importance of employing appropriate models to study EDC\u2010adipocyte interactions. Conclusions from this research could guide strategies to reduce the negative impacts of EDC exposure on adipose tissue.<\/jats:p>","DOI":"10.1002\/jat.4679","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T02:19:48Z","timestamp":1721787588000},"page":"1784-1792","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Endocrine disruption of adipose physiology: Screening in SGBS cells"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9917-936X","authenticated-orcid":false,"given":"Jan","family":"Kucera","sequence":"first","affiliation":[{"name":"RECETOX, Faculty of Science Masaryk University  Brno Czech Republic"},{"name":"Department of Physical Activities and Health Sciences, Faculty of Sports Studies Masaryk University  Brno Czech 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