{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T17:21:59Z","timestamp":1787851319747,"version":"build-2784847793"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T00:00:00Z","timestamp":1727395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260632"],"award-info":[{"award-number":["82260632"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cumulative evidence suggested that nanoplastics (NPs) cause male toxicity, but the mechanisms of which are still misty. Steroidogenesis is a key biological event that responsible for maintaining reproductive health. However, whether dysregulated steroidogenesis is involved in NPs\u2010induced impaired male reproductive function and the underlying mechanism remains unclear. In our study, Balb\/c mice were continuously exposed to pristine\u2010NPs or NH<jats:sub>2<\/jats:sub>\u2010NPs for 12\u2009weeks, spanning the puberty and adult stage. Upon the long\u2010term NPs treatment, the hypothalamus and testis were subjected to transcriptome and metabolome analysis. And the results demonstrated that both primitive\u2010NPs and NH<jats:sub>2<\/jats:sub>\u2010NPs resulted in impaired spermatogenesis and steroidogenesis, as evidenced by a significant reduction in sperm quality, testosterone, FSH, and LH. The expression of genes involved in hypothalamic\u2010pituitary\u2010testis (HPT) axis, such as <jats:italic>Kiss\u20101<\/jats:italic> and <jats:italic>Cyp17a1<\/jats:italic> that encoded the key steroid hormone synthetase, was also diminished. Furthermore, the phosphatidylcholine and pantothenic acid that mainly enriched in glycerophospholipid metabolism were significantly reduced in the testis. Comprehensive analysis of the transcriptome and metabolome indicated that down\u2010regulated <jats:italic>Cyp17a1<\/jats:italic> was associated with decreased metabolites phosphatidylcholine and pantothenic acid. Overall, we speculate that the disturbed HPT axis induced by long\u2010term NPs contributes to disordered glycerophospholipid metabolism and subsequently impaired steroidogenesis. Our findings deepen the understanding of the action of the mechanism responsible for NPs\u2010induced male reproductive toxicology.<\/jats:p>","DOI":"10.1002\/jat.4696","type":"journal-article","created":{"date-parts":[[2024,9,28]],"date-time":"2024-09-28T02:19:32Z","timestamp":1727489972000},"page":"298-310","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Long\u2010term nanoplastics exposure contributes to impaired steroidogenesis by disrupting the hypothalamic\u2010testis axis: Evidence from integrated transcriptome and metabolome analysis"],"prefix":"10.1002","volume":"45","author":[{"given":"Qian","family":"He","sequence":"first","affiliation":[{"name":"Department of Gynaecology and Obstetrics Affiliated Hospital of Zunyi Medical University  Zunyi Guizhou China"},{"name":"Key Laboratory of Anesthesia and Organ Protection of Ministry of Education (in cultivation) Zunyi Medical University  Zunyi Guizhou 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