{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T10:08:15Z","timestamp":1784542095410,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T00:00:00Z","timestamp":1726444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Polycystic ovary syndrome (PCOS) is a common endocrine disorder that can cause menstrual irregularities, infertility, polycystic ovaries, and metabolic abnormalities. Female reproductive health and quality of life are significantly affected by PCOS, which has recently been associated with ferroptosis in granulosa cells (GCs). Nuciferine (NF) is a naturally extracted substance with multiple pharmacological activities, which is reported with anti\u2010ferroptosis function. Herein, the influence of NF for androgen\u2010induced ferroptosis in GCs was investigated to explore the potential value of NF on treating PCOS. 10\u2009\u03bcM NF and 20\u2009\u03bcM NF were employed for treating KGN cells according to cell viability results. KGN cells were treated with 10\u2009\u03bcM dehydroepiandrosterone (DHEA) for 1\u2009day, followed by introducing 10\u2009\u03bcM NF and 20\u2009\u03bcM NF for 24\u2009h. Strikingly reduced cell viability, increased lactate dehydrogenase release and reactive oxygen species (ROS) production, enhanced apoptosis, upregulated Bax, downregulated Bcl\u20102, restrained malondialdehyde contents, and declined superoxide dismutase activity were observed in DHEA\u2010treated KGN cells, which were significantly reversed by NF. Significantly repressed GPX4, SLC7A11, and SOX2 levels, as well as increased ACSL4 levels and Fe<jats:sup>2+<\/jats:sup> levels in DHEA\u2010treated KGN cells, were notably rescued by NF. Furthermore, the inhibitory effect of NF on ROS production and ferroptosis in DHEA\u2010treated KGN cells was partially abrogated by silencing SOX2. Collectively, NF protected DHEA\u2010injured ovarian GCs by inhibiting ferroptosis via upregulating SOX2.<\/jats:p>","DOI":"10.1002\/jat.4697","type":"journal-article","created":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T14:09:25Z","timestamp":1726582165000},"page":"256-265","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Nuciferine protects hyperandrogen\u2010injured ovarian granulosa cells by inhibiting ferroptosis via SOX2\u2010mediated activation of the SLC7A11\/GPX4 axis"],"prefix":"10.1002","volume":"45","author":[{"given":"Hongyu","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Bethune International Peace Hospital  Shijiazhuang Hebei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shichao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Bethune International 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