{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:35:34Z","timestamp":1760574934500,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T00:00:00Z","timestamp":1733184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005047","name":"Natural Science Foundation of Liaoning Province","doi-asserted-by":"publisher","award":["2021\u2010MS\u2010355","2024\u2010MS\u2010203"],"award-info":[{"award-number":["2021\u2010MS\u2010355","2024\u2010MS\u2010203"]}],"id":[{"id":"10.13039\/501100005047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Ischemic heart disease remains a global health problem with high morbidity and mortality. Butorphanol, as a novel opioid, has been discovered with its cardioprotective properties. The purpose of this study was to explore that butorphanol can alleviate hypoxia\/reoxygenation (H\/R)\u2010induced myocardial injury by activating the Wnt\/\u03b2\u2010catenin signal pathway. In this study, Cell Counting Kit\u20108 (CCK\u20108), lactate dehydrogenase (LDH) kit, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, and Western blotting experiments were performed to observe butorphanol cardioprotective function and expression of Wnt signaling pathway proteins. For the main result, anti\u2010apoptotic effect and higher expression of Wnt signaling pathway proteins were observed with the increasing concentrations of butorphanol. Compared with the H\/R group, higher cellular viability, lower LDH release, and smaller apoptotic cell population were found in the butorphanol group. In addition, expression levels of apoptosis\u2010related protein Bax and Cleaved Caspase\u20103 decreased, and increased expression of Bcl\u20102 were observed. Conversely, the protective effects of butorphanol were attenuated in the XAV939 group. In summary, butorphanol attenuates hypoxia\/reoxygenation\u2010induced myocardial injury by activating the Wnt\/\u03b2\u2010catenin signal pathway. Our work provides a theoretical basis for butorphanol's myocardial protective function.<\/jats:p>","DOI":"10.1002\/jat.4736","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T21:04:02Z","timestamp":1733259842000},"page":"636-645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Butorphanol Alleviates Hypoxia\/Reoxygenation\u2010Induced Myocardial Injury by Activating Wnt\/\u03b2\u2010Catenin Signal Pathway"],"prefix":"10.1002","volume":"45","author":[{"given":"Shi\u2010Rui","family":"Hao","sequence":"first","affiliation":[{"name":"Department of Anesthesiology First Hospital Affiliated of Jinzhou Medical University  Jinzhou Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi\u2010Feng","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology First Hospital Affiliated of Jinzhou Medical University  Jinzhou Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia\u2010Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Second Outpatient Department of Traditional Chinese Medicine First Hospital Affiliated of Jinzhou Medical University  Jinzhou Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin\u2010Zhi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Thyroid Surgery First Hospital Affiliated of Jinzhou Medical University  Jinzhou Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting\u2010Ting","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Emergency Surgery First Hospital Affiliated of Jinzhou Medical University  Jinzhou Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tu","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology First Hospital Affiliated of Jinzhou Medical University  Jinzhou Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6342-6883","authenticated-orcid":false,"given":"Qiao\u2010Ling","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology First Hospital Affiliated of Jinzhou Medical University  Jinzhou Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112160"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23415"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450117666160401120308"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00022.2018"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.163.1.2005093"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102603"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3587"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12871\u2010022\u201001594\u20109"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01427"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjorl.2023.101369"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201803_14601"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2022.11151"},{"key":"e_1_2_11_14_1","first-page":"68","article-title":"Myocardial Necrosis Induced by Temporary Occlusion of a Coronary Artery in the Dog","volume":"70","author":"Jennings R. 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