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A total of 80 rats were randomly divided into four groups: control group and three doses of CTD groups. After 0, 1, 5, 15, and 28\u2009days of intragastric administration, rat serum and urine were collected for biochemical indexes, then serum was used for metabolomic analyses, and rat kidney was collected for pathological and ultrastructural observation. The levels of serum crea (Scr), blood urea nitrogen (BUN), urea, urine crea (Ucrea), and urinary microalbumin (UmALB) were significantly increased after administration of different doses of CTD (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Additionally, histopathology and cell ultrastructure observation of kidney showed significant cell inflammatory infiltration and glomerular edema. Seven metabolic biomarkers including 6\u2010hydroxymelatonin were significantly disturbed by CTD. The CatBoost Classifier prediction model was used to establish the CTD nephrotoxicity prediction model, and the prediction accuracy and precision were 0.645 and 0.640, respectively. Moreover, 6\u2010hydroxymelatonin was found to be most useful biomarkers for evaluating the CTD nephrotoxicity. Finally, the seven metabolic biomarkers were found mainly involved in pyruvate metabolism, pantothenate and CoA biosynthesis.<\/jats:p>","DOI":"10.1002\/jat.4743","type":"journal-article","created":{"date-parts":[[2024,12,15]],"date-time":"2024-12-15T23:10:02Z","timestamp":1734304202000},"page":"736-754","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Study of Serum Metabolic Biomarkers and Prediction Models of Cantharidin\u2010Induced Nephrotoxicity in Rats Based on Dynamic Metabolomics"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4963-6109","authenticated-orcid":false,"given":"Weina","family":"Cheng","sequence":"first","affiliation":[{"name":"School of Pharmacy Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0757-2779","authenticated-orcid":false,"given":"Wenzhong","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Pharmacy Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghuan","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Pharmacy Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingxian","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixun","family":"Bai","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Yan","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanmei","family":"He","sequence":"additional","affiliation":[{"name":"School of Pharmacy Zunyi Medical University  Zunyi China"},{"name":"Key Laboratory of Basic Pharmacology of Ministry Education and Joint International Research Laboratory of Ethnomedicine Ministry of Education Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofei","family":"Li","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9929-2768","authenticated-orcid":false,"given":"Jianyong","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Zunyi Medical University  Zunyi China"},{"name":"Key Laboratory of Basic Pharmacology of Ministry Education and Joint International Research Laboratory of Ethnomedicine Ministry of Education Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/japtr.JAPTR_336_18"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2021.105019"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2022.154231"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/7252280"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12987-023-00477-6"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2016.09.014"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ckj\/sft001"},{"key":"e_1_2_10_9_1","unstructured":"Dorogush A. 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