{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:48:46Z","timestamp":1760575726380,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:00:00Z","timestamp":1741651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Retinal toxicity is of great concern during drug development due to the irreversibility. Circulating microRNA (miRNA) is reported to be useful for detecting retinal toxicity in rats, although there has been no assessment of the diagnostic performance with statistical analysis. Therefore, we comparatively analyzed the diagnostic performance of circulating miRNAs enriched in the retina such as rno\u2010miR\u2010124\u20103p, \u2010183\u20105p, \u201096\u20105p, \u2010182, \u20109a\u20105p, \u2010125b\u20105p, \u2010204\u20105p and \u2010211\u20105p. The toxicants used in this study resulted in three types of retinal injury in photoreceptor (PR) cells, neuroretinal (NR) cells and retinal pigment epithelium (RPE) cells in rats. The performance of these biomarkers of retinal toxicity were assessed by receiver operator characteristic (ROC) analysis, then cut\u2010off values indicating the histopathological retinal lesions and performance indexes such as area under the ROC curve (ROC\u2010AUC), sensitivity and specificity were calculated. The ROC\u2010AUC for PR cell toxicity in relation to rno\u2010miR\u2010183\u20105p and \u2010182 were 0.970 and 0.873, respectively, and for NR cell toxicity in relation to rno\u2010miR\u2010124\u20103p it was 0.896. Our results suggest that plasma rno\u2010miR\u2010124\u20103p and \u2010183\u20105p\/\u2010182 would be suitable for detecting NR cell toxicity and PR cell toxicity, respectively. In conclusion, these plasma miRNAs may be an effective screening tool to detect drug\u2010induced retinal toxicity in preclinical toxicology studies.<\/jats:p>","DOI":"10.1002\/jat.4775","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T03:02:13Z","timestamp":1741748533000},"page":"1294-1304","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Diagnostic Performance of Circulating microRNAs as Biomarkers of Retinal Toxicity in the Rat"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3454-4772","authenticated-orcid":false,"given":"Daichi","family":"Ishii","sequence":"first","affiliation":[{"name":"Safety Research Department, Discovery Research Laboratories Nippon Shinyaku Co., Ltd.  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