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Synthetic JH agonists (JHAs), categorized as insect growth regulators, have been widely employed as insecticides. Natural JHs and synthetic JHAs both exert their physiological effects by binding to the JH receptor methoprene\u2010tolerant (Met), forming a functional heterodimer complex with steroid receptor coactivators (SRCs). These juvenoids induce male offspring production in various daphnids, including <jats:styled-content style=\"fixed-case\"><jats:italic>Daphnia magna<\/jats:italic><\/jats:styled-content>, highlighting the significance of the Met\u2010mediated signaling in environmental sex determination. As a representative invertebrate model for assessing aquatic endocrine\u2010disrupting chemicals, <jats:styled-content style=\"fixed-case\"><jats:italic>D. magna<\/jats:italic><\/jats:styled-content> is incorporated in the test guidelines of the Organization for Economic Corporation and Development. We herein introduced a newly developed yeast\u2010based reporter gene assay (RGA) for easy and rapid screening of JH\u2010like ligands for <jats:styled-content style=\"fixed-case\"><jats:italic>D. magna<\/jats:italic><\/jats:styled-content> Met (Dapma\u2010Met). Dapma\u2010Met was expressed alongside the SRC of <jats:styled-content style=\"fixed-case\"><jats:italic>D. magna<\/jats:italic><\/jats:styled-content> (Dapma\u2010SRC) in yeast cells carrying the <jats:italic>lacZ<\/jats:italic> reporter plasmid with a JH\u2010responsive element derived from the <jats:italic><jats:styled-content style=\"fixed-case\">Bombyx mori<\/jats:styled-content> Kr\u00fcppel homolog 1<\/jats:italic> gene. The yeast RGA system for Dapma\u2010Met revealed a dose\u2010dependent response to various juvenoids. The rank order of the ligand potencies of natural JHs and synthetic JHAs examined in yeast RGA strongly correlated with those previously observed in RGAs for <jats:italic>Daphnia<\/jats:italic> Met proteins established in Chinese hamster ovary cells and positively correlated with the male neonate\u2010inducing activity in\u00a0vivo. Our novel yeast RGA offers a rapid, easy\u2010to\u2010handle, and cost\u2010effective solution that will be valuable for discriminating Dapma\u2010Met ligands among chemicals with male offspring\u2010inducing activity.<\/jats:p>","DOI":"10.1002\/jat.4784","type":"journal-article","created":{"date-parts":[[2025,4,13]],"date-time":"2025-04-13T23:18:46Z","timestamp":1744586326000},"page":"1496-1508","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a New Reporter Gene Assay for Detecting Juvenile Hormone Agonists Using Yeast Expressing Methoprene\u2010Tolerant of the Freshwater Cladoceran <scp><i>Daphnia magna<\/i><\/scp>"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8884-7937","authenticated-orcid":false,"given":"Sayoko","family":"Ito\u2010Harashima","sequence":"first","affiliation":[{"name":"Department of Biological Science, Graduate School of Science Osaka Prefecture University  Sakai Osaka Japan"},{"name":"Department of Applied Biological Chemistry, Graduate School of Agriculture Osaka Metropolitan University  Sakai Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"Sano","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Graduate School of Science Osaka Prefecture University  Sakai Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiji","family":"Takada","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Graduate School of Science Osaka Prefecture University  Sakai Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayuko","family":"Nakashima","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, Graduate School of Science Osaka Metropolitan University  Sakai Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanobu","family":"Kawanishi","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Graduate School of Science Osaka Prefecture University  Sakai Osaka Japan"},{"name":"Department of Biological Chemistry, Graduate School of Science Osaka Metropolitan University  Sakai Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5476-8424","authenticated-orcid":false,"given":"Takashi","family":"Yagi","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Graduate School of Science Osaka Prefecture University  Sakai Osaka Japan"},{"name":"Department of Biological Chemistry, Graduate School of Science Osaka Metropolitan University  Sakai Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,13]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_10_2_1","DOI":"10.1016\/j.aquatox.2014.11.015"},{"doi-asserted-by":"publisher","key":"e_1_2_10_3_1","DOI":"10.1016\/B978-0-12-374144-8.00155-7"},{"doi-asserted-by":"publisher","key":"e_1_2_10_4_1","DOI":"10.1074\/jbc.RA118.005992"},{"volume-title":"Methods in Yeast Genetics\u2014A Laboratory Course Manual, Cold Spring Harbor","year":"2000","author":"Burke D.","key":"e_1_2_10_5_1"},{"doi-asserted-by":"publisher","key":"e_1_2_10_6_1","DOI":"10.1016\/S0269\u20107491(97)00030\u20104"},{"doi-asserted-by":"publisher","key":"e_1_2_10_7_1","DOI":"10.1002\/tox.20473"},{"unstructured":"Chung T. 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