{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:18:52Z","timestamp":1784128732716,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Iohexol is a nonionic contrast agent used in x\u2010ray imaging but can sometimes cause anaphylactic reactions. The mechanism of anaphylaxis\u2010induced fatal respiratory symptoms remains unclear. The aim of this study was to investigate the effects of iohexol on cytotoxicity and DNA damage in bronchial epithelial cells (BEAS\u20102B). BEAS\u20102B cells were treated with Iohexol (1.5, 15, 75, and 150\u2009mg I\/mL) and cell viability was examined by 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyl\u20102H\u2010tetrazolium bromide (MTT) assay. The IC\n                    <jats:sub>50<\/jats:sub>\n                    value of iohexol was determined and the DNA damage effect of iohexol (12.4, 24.9, 49.9, and 99.9\u2009mM) on BEAS\u20102B cells was investigated by Comet assay. Molecular docking analysis was also performed to determine the binding affinity to DNA and the antiapoptotic protein BCL\u2010XL. Iohexol 15\u2009mg I\/mL, iohexol 75\u2009mg I\/mL, and iohexol 150\u2009mg I\/mL groups significantly decreased cell viability compared to the control group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). Iohexol (12.4, 24.9, 49.9, and 99.9\u2009mM) did not significantly alter genotoxicity in BEAS\u20102B cells compared to the control group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). In silico study showed that iohexol binds with high affinity to BCL\u2010XL but low affinity to DNA. Our results show that iohexol causes cytotoxicity in BEAS\u20102B cells and binds strongly to BCL\u2010XL. Our results suggest that the safety of iohexol needs further detailed studies.\n                  <\/jats:p>","DOI":"10.1002\/jat.4800","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T21:29:03Z","timestamp":1745962143000},"page":"1686-1693","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["In Vitro Cytogenotoxicity of Iohexol in Bronchial Epithelial Cells and In Silico DNA and BCL\u2010XL\n                    <i>Affinity<\/i>\n                    Studies"],"prefix":"10.1002","volume":"45","author":[{"given":"Ibrahim\u00a0Halil","family":"Kenger","sequence":"first","affiliation":[{"name":"Department of Medical Genetics, Faculty of Medicine Gaziantep Islam Science and Technology University  Gaziantep Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3470-1783","authenticated-orcid":false,"given":"Batuhan","family":"Bilgin","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Faculty of Medicine Gaziantep Islam Science and Technology University  Gaziantep Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12640-023-00678-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00120804"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004424-199405001-00007"},{"issue":"1","key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"6","DOI":"10.14302\/issn.2574-4488.jna-14-601","article-title":"The Toxicity of Iodinated Radiographic Contrast Agents in the Clinical Practice","volume":"1","author":"Andreucci M.","year":"2015","journal-title":"Journal of Nephrology Advances"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90161-2"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2023.166932"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0033-1348885"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinimag.2016.04.008"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-005-0051-7"},{"key":"e_1_2_9_11_1","volume-title":"The Role of DNA\u2010Fragmentation in Contrast\u2010Induced Tubular Cell Damage","author":"Dikopoulos J. 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