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Following our previous studies, for the ex\u00a0vivo short\u2010term effect, we chose 10\n                    <jats:sup>\u22129<\/jats:sup>\n                    \u2009M pyriproxyfen to analyze the morphology of spermatogenesis wave cells. In silico docking and ADMET (Absorption, Distribution, Metabolism, Excretion, Toxicity) studies were carried out to preliminarily predict possible interaction modes between PPX and the T\u2010type voltage\u2010dependent calcium channel (T\u2010VDCC), as well as with protein kinase C (PKC), as we previously reported by using pharmacological approach. The in silico ADMET evaluations revealed that PPX demonstrates notable lipophilicity. Moreover, PPX is predicted to inhibit the enzymatic activity of CYP1A2, CYP2C19, CYP2C9, and CYP2D6. Furthermore, in silico molecular docking analyses revealed that PPX has the potential to interact with the T\u2010VDCC through hydrogen bonds with Gln1653 and hydrophobic interactions with Leu291, Phe322, Phe1607, and Leu1656. Possible interactions of PPX with PKC involve ionic bonding with Lys463, hydrogen bonds with His592, and hydrophobic interactions with Lys463, Val596, Gly591, Phe593, Lys611, Asp711, and Leu714 reinforcing these both targets to PPX. In summary, short\u2010term PPX exposure influenced the morphology of testicular cells (spermatids, spermatozoa, and Leydig cells) through interactions with molecular targets. Findings reveal the bimodal effects (on morphology and signaling) of this compound on specific cells within the spermatogenic wave, endocrine cells, and signal transduction proteins. This interference may impair reproduction and lead to male infertility. In addition, the prediction from both molecular docking and ADMET supported our in\u00a0vitro mechanistic analysis firstly reported in the testis of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Danio rerio<\/jats:italic>\n                    <\/jats:styled-content>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/jat.4801","type":"journal-article","created":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T05:10:27Z","timestamp":1746076227000},"page":"1705-1719","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Pyriproxyfen Disrupts the Ongoing Spermatogenesis Wave in\n                    <scp>\n                      <i>Danio rerio<\/i>\n                    <\/scp>\n                    Potentially Mediated by Voltage\u2010Dependent Calcium Channels and Protein Kinase C"],"prefix":"10.1002","volume":"45","author":[{"given":"Bruna\u00a0Antunes","family":"Zaniboni","sequence":"first","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL): Ci\u00eancia &amp; Sa\u00fade Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"},{"name":"Departamento de Bioqu\u00edmica, Centro de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa\u00a0Staldoni\u00a0de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL): Ci\u00eancia &amp; Sa\u00fade Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"},{"name":"Departamento de Bioqu\u00edmica, Centro de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel\u00a0Adan\u00a0Araujo","family":"Leite","sequence":"additional","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL): Ci\u00eancia &amp; Sa\u00fade Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"},{"name":"Departamento de Biologia Celular, Embriologia e Gen\u00e9tica, Centro de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valdel\u00facia\u00a0Maria\u00a0Alves\u00a0de\u00a0Souza","family":"Grinevicius","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Centro de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rozangela\u00a0Curi","family":"Pedrosa","sequence":"additional","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL): Ci\u00eancia &amp; Sa\u00fade Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"},{"name":"Departamento de Bioqu\u00edmica, Centro de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1391-2354","authenticated-orcid":false,"given":"F\u00e1tima\u00a0Regina\u00a0Mena\u00a0Barreto","family":"Silva","sequence":"additional","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL): Ci\u00eancia &amp; Sa\u00fade Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"},{"name":"Departamento de Bioqu\u00edmica, Centro de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.113.113555"},{"key":"e_1_2_10_3_1","unstructured":"Ag\u00eancia de Defesa Agropecu\u00e1ria do Paran\u00e1 (ADAPAR).2021.Piriproxifen Insecticide: Recommendations and Use in Agricultural Crops. 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