{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T16:27:45Z","timestamp":1767025665637,"version":"3.48.0"},"reference-count":47,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["20201J4082"],"award-info":[{"award-number":["20201J4082"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Beryllium and its compounds are classified as carcinogens, and prolonged exposure can trigger chronic beryllium disease. The Janus kinase\u2013signal transducer and activator of transcription (JAK\u2010STAT) signaling is known to play a critical role in the development and progression of numerous diseases. Leukemia inhibitory factor (LIF), a key upstream cytokine of the JAK\u2010STAT pathway, has been implicated in regulating inflammatory responses and epithelial\u2010mesenchymal transition (EMT) in various diseases. However, the specific involvement of the JAK\u2010STAT pathway and LIF in beryllium sulfate (BeSO\u2084)\u2013induced EMT in human bronchial epithelial (16HBE) cells remains unclear. To investigate the regulatory mechanisms, we examined the effects of BeSO\u2084 on 16HBE cells and targeted the JAK\u2010STAT pathway using both pharmacological inhibition (niclosamide) and genetic silencing of LIF. Subsequently, we assessed cell morphology, proliferative capacity, inflammatory protein levels, and EMT marker expression. Our findings demonstrated that BeSO\u2084 exposure inhibited 16HBE cell proliferation and activated the JAK\u2010STAT pathway. Pretreatment with niclosamide significantly mitigated cellular inflammation and the EMT process induced by BeSO\u2084. Additionally, silencing LIF markedly reduced JAK\u2010STAT pathway activation and decreased the expression of EMT markers. This study uncovers a novel mechanism underlying BeSO\u2084\u2010induced EMT in 16HBE cells, providing valuable insights into the molecular mechanisms of toxicity induced by beryllium and its compounds.<\/jats:p>","DOI":"10.1002\/jat.4802","type":"journal-article","created":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T21:56:36Z","timestamp":1746136596000},"page":"1720-1729","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Leukemia Inhibitory Factor via JAK\u2010STAT Signaling Drives Beryllium Sulfate\u2013Induced Epithelial\u2013Mesenchymal Transition in 16HBE Cells"],"prefix":"10.1002","volume":"45","author":[{"given":"Yaqi","family":"Li","sequence":"first","affiliation":[{"name":"Department of Preventive Medicine, School of Public Health, Hengyang Medical School University of South China  Hengyang 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