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This study aimed to study the acute in\u00a0vitro toxicity of ethylbenzene and\n                    <jats:italic>m<\/jats:italic>\n                    \u2010xylene on human bronchial epithelial cells exposed at the air\u2013liquid interface (ALI). The cells were exposed to VOCs alone and in a mixture for 1\u2009h, followed by 5, 23, and 47\u2009h of incubation. The kinetics of the cell response was characterized, including cytotoxicity, xenobiotic biotransformation, antioxidant defense system, inflammatory response, and apoptosis. The gene expression results showed major differences between these two compounds, even though their chemical structure is very similar. Ethylbenzene did not appear to be metabolized in BEAS\u20102B cells, as it inhibited gene expression of xenobiotic metabolizing enzymes (XME) and did not induce antioxidant defense systems or apoptosis. However, a slight inflammatory response was observed after exposure.\n                    <jats:italic>m<\/jats:italic>\n                    \u2010Xylene was metabolized in BEAS\u20102B cells, inducing several XMEs and upregulating enzymes involved in the antioxidant defense system, as well as markers of inflammation and apoptosis. Co\u2010exposure to the binary mixture resulted in an inhibition phenomenon, resulting in the inhibition of toxic action mechanisms studied. The results provide new information on the toxicity of ethylbenzene and\n                    <jats:italic>m<\/jats:italic>\n                    \u2010xylene and highlight the importance of conducting ALI exposures to mixtures of toxicants.\n                  <\/jats:p>","DOI":"10.1002\/jat.4806","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T01:59:28Z","timestamp":1746755968000},"page":"1770-1786","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Toxicological Response of the BEAS\u20102B Cell After Acute Exposure at the Air\u2013Liquid Interface to Ethylbenzene and\n                    <i>m<\/i>\n                    \u2010Xylene Alone and in Binary Mixtures"],"prefix":"10.1002","volume":"45","author":[{"given":"Nour","family":"Jaber","sequence":"first","affiliation":[{"name":"UR4492, Unit\u00e9 de Chimie Environnementale et Interactions sur le Vivant Universit\u00e9 du Littoral 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