{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:05:31Z","timestamp":1787508331580,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2020JJ4082"],"award-info":[{"award-number":["2020JJ4082"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Beryllium (Be) is a recognised environmental toxicant associated with pulmonary fibrosis. Epithelial\u2013mesenchymal transition (EMT), a critical process in cell phenotype conversion, plays a key role in its pathophysiology. Methyltransferase\u2010like 3 (METTL3), a major N6\u2010methyladenosine methyltransferase, regulates gene expression and cellular functions. However, its role in Be\u2010induced EMT remains unclear. In this study, human bronchial epithelial cell line (16HBE cells) were exposed to varying concentrations of beryllium sulphate (BeSO\n                    <jats:sub>4<\/jats:sub>\n                    ) to assess changes in METTL3 expression. METTL3 overexpression vectors were constructed, and quantitative reverse transcription\u2010polymerase chain reaction, western blotting and immunofluorescence were used to detect METTL3, EMT markers and Wingless\/Integrated (Wnt)\/\u03b2\u2010catenin pathway proteins. The Wnt\/\u03b2\u2010catenin pathway inhibitor ICG\u2010001 was also employed to explore the role of the Wnt\/\u03b2\u2010catenin pathway in BeSO\n                    <jats:sub>4<\/jats:sub>\n                    \u2010induced EMT. The study demonstrated that BeSO\n                    <jats:sub>4<\/jats:sub>\n                    suppressed METTL3 expression, induced EMT and activated the Wnt\/\u03b2\u2010catenin pathway in 16HBE cells. Both METTL3 overexpression and ICG\u2010001 pretreatment mitigated BeSO\n                    <jats:sub>4<\/jats:sub>\n                    \u2010induced EMT and Wnt\/\u03b2\u2010catenin pathway activation. These findings suggest that METTL3 inhibits BeSO\n                    <jats:sub>4<\/jats:sub>\n                    \u2010induced EMT by suppressing the Wnt\/\u03b2\u2010catenin pathway, offering novel mechanistic insights into beryllium toxicity and a potential therapeutic target for Be\u2010related pulmonary fibrosis.\n                  <\/jats:p>","DOI":"10.1002\/jat.4807","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T01:39:31Z","timestamp":1746754771000},"page":"1787-1797","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["METTL3 Mediates Wnt\/\u03b2\u2010Catenin Pathway in Epithelial\u2013Mesenchymal Transition of 16HBE Cells Induced by Beryllium Sulphate"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3378-7305","authenticated-orcid":false,"given":"Chenxi","family":"Yan","sequence":"first","affiliation":[{"name":"Department of 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