{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T12:59:24Z","timestamp":1775134764701,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T00:00:00Z","timestamp":1747872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\u03b1\u2010Terpineol, a monoterpene found in essential oils and fragrances, has been reported to exhibit reproductive toxicity. The present study investigates the impact of oxidative stress on reproductive toxicity induced by \u03b1\u2010Terpineol in male rats. \u03b1\u2010Terpineol was administered to rats at various doses (0, 75, 150, and 300\u2009mg\/kg\/day) via oral gavage for 28\u2009days. Endpoints assessed included sperm parameters, testicular histopathology, morphology, and the expression of Nrf2 a key regulator of cellular antioxidant defense, using immunohistochemistry, serum hormone analysis, and markers of oxidative stress in the testes malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), ferric reducing antioxidant power (FRAP) assay, and protein carbonyl content (PC) assay. Results demonstrated that \u03b1\u2010Terpineol significantly induced oxidative stress as evidenced by increased MDA levels and decreased SOD, GSH, CAT, activities, and FRAP level. Furthermore, \u03b1\u2010Terpineol exposure resulted in a dose\u2010dependent reduction in sperm motility and concentration, increased abnormal sperm morphology, decreased serum testosterone, FSH and LH levels, and histopathological alterations in the testes indicating disruption of the hypothalamic\u2013pituitary\u2013gonadal axis. These findings suggest that oxidative stress plays a crucial role in \u03b1\u2010Terpineol\u2010induced reproductive toxicity in male rats, potentially through disruption of antioxidant defense mechanisms and subsequent damage to testicular tissues and sperm.<\/jats:p>","DOI":"10.1002\/jat.4815","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T01:45:44Z","timestamp":1747964744000},"page":"1887-1899","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of Oxidative Stress on Reproductive Toxicity Induced by Alpha\u2010Terpineol in Male Sprague\u2013Dawley Rats"],"prefix":"10.1002","volume":"45","author":[{"given":"Pakkiresha","family":"Goravara","sequence":"first","affiliation":[{"name":"Reproductive Biology and Mechanistic Toxicology Lab, Department of Studies in Zoology Vijayanagara Sri Krishnadevaraya University  Ballari India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3338-1028","authenticated-orcid":false,"given":"Vijaykumar\u00a0B.","family":"Malashetty","sequence":"additional","affiliation":[{"name":"Reproductive Biology and Mechanistic Toxicology Lab, Department of Studies in Zoology Vijayanagara Sri Krishnadevaraya University  Ballari India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,22]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"121","volume-title":"Methods in Enzymology","author":"Aebi H.","year":"1984"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.5534\/wjmh.2014.32.1.1"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/jhrs.JHRS_150_18"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2023.114147"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204\u2010011\u20100674\u20105"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1201\/b19393"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/3527600205"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2018.02.010"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0292"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s000110050746"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi973101r"},{"issue":"1","key":"e_1_2_11_13_1","first-page":"37","article-title":"Effects of Dietary Magnesium on Testicular Histology, Steroidogenesis, Spermatogenesis and Oxidative Stress Markers in Adult Rats","volume":"51","author":"Chandra A. 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