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The major constituents found in fennel seed extracts are trans\u2010anethole (68.6%\u201375.0%), fenchone (8.40%\u201314.7%), and methyl chavicol (5.09%\u20139.10%). However, before introducing this plant into the human environment, it is essential to understand its toxicological properties. In this study, we investigated the acute and sub\u2010acute toxicity of methanolic extracts of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>F.\u2009vulgare<\/jats:italic>\n                    <\/jats:styled-content>\n                    . A phytochemical screening was performed to identify the major chemical constituents of the plant. For the acute toxicity study, female Wistar rats received a single dose of the methanolic extract of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>F.\u2009vulgare<\/jats:italic>\n                    <\/jats:styled-content>\n                    at doses of 1000, 2000, 3000, and 5000\u2009mg\/kg for 14\u2009days. In the sub\u2010acute toxicity study, the methanolic extract of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>F.\u2009vulgare<\/jats:italic>\n                    <\/jats:styled-content>\n                    was administered orally daily at doses of 125, 250, and 500\u2009mg\/kg for 28\u2009days. Hematological, biochemical, and histological changes were evaluated. Phytochemical tests were also performed. The phytochemical analysis showed that the methanolic extract of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>F.\u2009vulgare<\/jats:italic>\n                    <\/jats:styled-content>\n                    is rich in flavonoids, catechic, gallic tannins, and total polyphenols. Toxicological tests showed no animal deaths, suggesting that the LD\n                    <jats:sub>50<\/jats:sub>\n                    was greater than 5000\u2009mg\/kg. In the sub\u2010acute oral toxicity study, no significant differences were observed in body weight, food consumption, or water intake. Additionally, there were no significant changes in hematological and biochemical parameters or differences in the macroscopic and microscopic examination of organs. Therefore, this study concludes that the methanolic extract of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>F.\u2009vulgare<\/jats:italic>\n                    <\/jats:styled-content>\n                    , at the doses tested, is considered non\u2010toxic under the conditions evaluated.\n                  <\/jats:p>","DOI":"10.1002\/jat.4907","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T03:39:23Z","timestamp":1758080363000},"page":"877-885","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Assessing Acute and Subacute Toxicity and Phytochemical Screening of the Methanolic Extract of\n                    <scp>\n                      <i>Foeniculum\u2009vulgare<\/i>\n                    <\/scp>\n                    in Wistar 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