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It is primarily absorbed into the systemic circulation via the gastrointestinal tract, which may damage the brush border membrane (BBM) and intestinal mucosal barrier, leading to numerous adverse health effects. Therefore, the present in\u00a0vivo and in\u00a0vitro studies were carried out to evaluate the effect of aluminum chloride (AlCl\n                    <jats:sub>3<\/jats:sub>\n                    ) treatment on oxidative stress parameters, cellular metabolism, membrane integrity, and DNA damage in the rat intestine. For in\u00a0vivo studies, adult male Wistar rats were randomly divided into five groups, namely, the control group (C group) and AlCl\n                    <jats:sub>3<\/jats:sub>\n                    \u2010treated groups: Al I (25\u2009mg\/kg b.wt.), Al II (35\u2009mg\/kg b.wt.), Al III (45\u2009mg\/kg b.wt.), and Al IV (55\u2009mg\/kg b.wt.). A dose\u2010dependent decline in the specific activities of BBM marker enzymes such as leucine aminopeptidase (LAP), alkaline phosphatase (ALP), \u03b3\u2010glutamyltransferase (GGTase), and sucrase was detected, both in the mucosal homogenates and in the isolated membrane vesicles. Vmax values of the enzymes exhibited a significant decline following treatment, whereas K\n                    <jats:sub>M<\/jats:sub>\n                    values remained unaltered. Furthermore, AlCl\n                    <jats:sub>3<\/jats:sub>\n                    administration at all four doses altered the specific activities of energy metabolism enzymes and perturbed the cellular antioxidant status, as apparent by a significant decline in endogenous enzymatic and nonenzymatic antioxidants and enhanced lipid peroxidation (LPO). Tail migration in a single\u2010cell gel electrophoresis indicated extensive DNA damage in mucosal cells in all the AlCl\n                    <jats:sub>3<\/jats:sub>\n                    \u2010treated groups; however, maximum damage was observed in rats administered AlCl\n                    <jats:sub>3<\/jats:sub>\n                    at a dose of 55\u2009mg\/kg b.wt. In\u00a0vitro incubation of BBM vesicles with AlCl\n                    <jats:sub>3<\/jats:sub>\n                    (0.1\u20133\u2009mM, 0\u2013120\u2009min) confirmed a concentration\u2010 and time\u2010dependent inhibition of BBM enzyme activities. Histological investigations revealed impaired intestinal histology concomitant with biochemical alterations. The present results indicate that aluminum\u2010induced oxidative stress produced substantial intestine damage, leading to altered levels of BBM, antioxidants, and carbohydrate metabolism enzymes, potentially guiding the development of protective strategies to mitigate its adverse health effects.\n                  <\/jats:p>","DOI":"10.1002\/jat.4910","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T06:10:39Z","timestamp":1756879839000},"page":"622-638","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Oral Administration of Aluminum Chloride Inhibits Enzymes of Brush Border Membrane and Induces Metabolic Distress, Redox Imbalance, DNA Damage, and Histopathologic Alterations in Rat Intestine"],"prefix":"10.1002","volume":"46","author":[{"given":"Samina","family":"Naseem","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Life Sciences Aligarh Muslim University  Aligarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farha","family":"shahabuddin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Life Sciences Aligarh Muslim University  Aligarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tauseef","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Life Sciences Aligarh Muslim University  Aligarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aijaz\u00a0Ahmed","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Faculty of Medicine, J.N. 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