{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T22:41:33Z","timestamp":1790116893925,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Acute lung injury (ALI) poses a significant threat in respiratory diseases and can lead to organ failure in chronic conditions. In this study, we evaluated the preventive effects of Dehydrozingerone\u20106 against Lipopolysaccharide (LPS)\u2010induced inflammation in macrophages using RAW 264.7 and in a mouse model of ALI. During preliminary screening, Dehydrozingerone\u20106 demonstrated significant inhibition of cytokines and maintained 97% cell viability at 10\u2009\u03bcM. Further in\u00a0vitro studies showed that Dehydrozingerone\u20106 effectively suppressed LPS\u2010induced production of nitric oxide, interleukin\u20106 (IL\u20106), tumor necrosis factor\u2010alpha (TNF\u2010\u03b1), interferon\u2010gamma (IFN\u2010\u03b3), interleukin\u20101 beta (IL\u20101\u03b2), and reactive oxygen species (ROS) at a concentration of 10\u2009\u03bcM. Immunocytochemistry and Western blot analyses revealed reduced expression levels of inducible nitric oxide synthase (iNOS), cyclooxygenase\u20102 (COX\u20102), Nuclear Factor kappa B (NF\u2010\u03baB\/p65), and phosphorylated I\u03baB\u03b1 (p\u2010I\u03baB\u03b1). In\u00a0vivo LPS\u2010induced lung injury model revealed that Dehydrozingerone\u20106 protected lung tissue from degradation and significantly inhibited IL\u20106 and TNF\u2010\u03b1 production at a dose of 50\u2009mg\/kg. Furthermore, Dehydrozingerone\u20106 significantly reduced carrageenan\u2010induced paw edema and leukocyte migration. Dehydrozingerone\u20106 also markedly reduced vascular permeability in an acetic acid\u2010induced model. Toxicological studies conducted at doses up to 2000\u2009mg\/kg body weight showed no notable alterations in hematological parameters or histopathology of the liver and kidney, suggesting a favorable safety profile. Overall, Dehydrozingerone\u20106 exhibited strong anti\u2010inflammatory activity in\u00a0vitro as well as in\u00a0vivo studies by downregulating the generation of pro\u2010inflammatory mediators and oxidative stress through attenuation of iNOS, COX\u20102, and NF\u2010\u03baB\/p65.<\/jats:p>","DOI":"10.1002\/jat.4943","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T07:27:25Z","timestamp":1759217245000},"page":"992-1006","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Anti\u2010Inflammatory Activity of Novel Dehydrozingerone\u20106 (DHZ\u20106) Through Modulation of iNOS, COX\u20102, and NF\u2010\u03baB\/p65 in LPS\u2010Stimulated Macrophages and In\u00a0Vivo Acute Lung Injury Murine Model"],"prefix":"10.1002","volume":"46","author":[{"given":"Narendra","family":"Chauhan","sequence":"first","affiliation":[{"name":"CSIR\u2010 Indian Institute of Integrative Medicine  Jammu 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