{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:47:26Z","timestamp":1772999246582,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T00:00:00Z","timestamp":1759622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T00:00:00Z","timestamp":1759622400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mercury is a heavy metal that naturally occurs in the environment or may be released through anthropogenic activities. Its toxicological impacts are well documented. Prolactin (PRL) is a hormone involved in several physiological processes, including metabolic regulation and tissue protection. This study aimed to investigate the potential protective effect of PRL against testicular histopathological damage induced by methylmercury (MeHg) exposure in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Mus musculus<\/jats:italic>\n                    <\/jats:styled-content>\n                    mice. The animals were allocated into six experimental groups: negative control, MeHg only, two groups treated with different PRL concentrations, and two groups receiving combined MeHg and PRL treatments. Testicular tissues were fixed, embedded in paraffin, sectioned using a microtome, stained with hematoxylin and eosin, and evaluated by light microscopy. Histopathological alterations observed included seminiferous tubule degeneration, cytoplasmic vacuolization, epithelial desquamation, and abnormal secretion. Statistical analysis was performed using a nonparametric ANOVA (Kruskal\u2013Wallis), with post hoc SNK test. The findings confirmed the testicular toxicity of MeHg and suggested a potential protective role of PRL, although PRL alone also demonstrated some toxicological effects. These results underscore the need for further research to elucidate the dual role of PRL in testicular toxicology.\n                  <\/jats:p>","DOI":"10.1002\/jat.4950","type":"journal-article","created":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T04:54:24Z","timestamp":1759726464000},"page":"1175-1181","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vivo Evaluation of the Potential Protective Effect of Prolactin Against Histological Damage Caused by Methylmercury in Testicles of Mice (\n                    <scp>\n                      <i>Mus musculus<\/i>\n                    <\/scp>\n                    )"],"prefix":"10.1002","volume":"46","author":[{"given":"Evandro","family":"da Concei\u00e7\u00e3o\u00a0Ara\u00fajo","sequence":"first","affiliation":[{"name":"Federal Institute of Education, Science and Technology of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9\u00a0Matheus\u00a0Almeida","family":"da Silva","sequence":"additional","affiliation":[{"name":"Federal Institute of Education, Science and Technology of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria\u00a0Luiza\u00a0Cunha","family":"e Souza\u00a0Ferreira","sequence":"additional","affiliation":[{"name":"Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f4nica\u00a0Regina\u00a0Lobato","family":"de Oliveira\u00a0Bahia","sequence":"additional","affiliation":[{"name":"Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3037-1323","authenticated-orcid":false,"given":"Carlos\u00a0Alberto\u00a0Machado","family":"da Rocha","sequence":"additional","affiliation":[{"name":"Federal Institute of Education, Science and Technology of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1085-3635","authenticated-orcid":false,"given":"Lorena\u00a0Ara\u00fajo","family":"da Cunha","sequence":"additional","affiliation":[{"name":"Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,5]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Bioestat 5.0: aplica\u00e7\u00f5es estat\u00edsticas nas \u00e1reas das ci\u00eancias biol\u00f3gicas e medicas","author":"Ayres M.","year":"2007"},{"issue":"4","key":"e_1_2_8_3_1","first-page":"51","article-title":"Efeitos Biol\u00f3gicos do Merc\u00fario e Seus Derivados em Seres Humanos: Uma Revis\u00e3o Bibliogr\u00e1fica","volume":"15","author":"Cardoso P. 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N.","year":"2014"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.4950","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jat.4950","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.4950","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:07:29Z","timestamp":1772989649000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.4950"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,5]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/jat.4950"],"URL":"https:\/\/doi.org\/10.1002\/jat.4950","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"value":"0260-437X","type":"print"},{"value":"1099-1263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,5]]},"assertion":[{"value":"2025-08-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}