{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T13:19:29Z","timestamp":1788355169370,"version":"build-2803163510"},"reference-count":62,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC3302002"],"award-info":[{"award-number":["2022YFC3302002"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    1,2\u2010Dichloroethane (1,2\u2010DCE), a common industrial organic solvent, enters humans primarily via inhalation, damaging the central nervous system and organs like the liver and kidneys. However, its cardiotoxicity and the multi\u2010organ developmental toxicity in zebrafish remain unclear. This study investigated its cardiotoxicity, hepatotoxicity, and nephrotoxicity using a zebrafish model. Zebrafish embryos at 4\u2009h after fertilization (hpf) were exposed to 0, 200, 400, or 800\u2009\u03bcg\/mL 1,2\u2010DCE until 120 hpf, and morphological and genetic changes were assessed. As the dosage of 1,2\u2010DCE increases, the mortality rates of zebrafish also increase (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), and the LC\n                    <jats:sub>50<\/jats:sub>\n                    was determined as 867.5\u2009\u03bcg\/mL. Exposure to 1,2\u2010DCE significantly increased pericardial area, heart rate, and sinus venosus\u2010bulbus arteriosus (SV\u2010BA) distance, upregulated\n                    <jats:italic>nkx2.5<\/jats:italic>\n                    and\n                    <jats:italic>gata4<\/jats:italic>\n                    , and downregulated\n                    <jats:italic>bmp4<\/jats:italic>\n                    , c\n                    <jats:italic>mlc1<\/jats:italic>\n                    ,\n                    <jats:italic>vmhc<\/jats:italic>\n                    , and\n                    <jats:italic>spaw<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). In the exposure groups, hepatic vacuolation and atrophy were observed, accompanied by downregulated\n                    <jats:italic>cyp1a<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Renal tubule length decreased dose\u2010dependently, with downregulation of\n                    <jats:italic>pax2a<\/jats:italic>\n                    ,\n                    <jats:italic>pax8<\/jats:italic>\n                    ,\n                    <jats:italic>wt1a<\/jats:italic>\n                    ,\n                    <jats:italic>wt1b<\/jats:italic>\n                    , and\n                    <jats:italic>nphsl<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) in the exposure group. These data demonstrate that 1,2\u2010DCE induces multi\u2010organ toxicity in zebrafish, causing structural and functional impairments in the heart, liver, and kidney. This suggests that the harmful effects of 1,2\u2010DCE on zebrafish should be taken into account when evaluating environmental risks in the future.\n                  <\/jats:p>","DOI":"10.1002\/jat.4976","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T07:27:15Z","timestamp":1762414035000},"page":"1593-1603","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Developmental Multi\u2010Organ Toxicity in Zebrafish Exposed to 1, 2\u2010Dichloroethane"],"prefix":"10.1002","volume":"46","author":[{"given":"Yang","family":"Xiang","sequence":"first","affiliation":[{"name":"Department of Occupational Health and Occupational Medicine, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyi","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Occupational Medicine, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Pang","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Occupational Medicine, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoshun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Occupational Medicine, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiazhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Occupational Medicine, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichang","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform Academy of Forensic Science  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningguo","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform Academy of Forensic Science  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Occupational Medicine, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00402468"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-003-0259-6"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2023.10.022"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.240102"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.103416"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2015.02.020"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.70241"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000015273"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ntt.2012.08.006"},{"key":"e_1_2_8_11_1","first-page":"391","volume-title":"Methods in Cell Biology","author":"Drummond I. 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