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The present study focuses on investigating the protective effects of fisetin, a bioactive flavonoid, in alleviating MeHg\u2010induced nephrotoxicity in offspring. Pregnant Wistar rats of gestational day 0 (GD 0) were divided into six groups, including control, 20\u2009mg\/kg fisetin, 0.5\u2009mg\/kg MeHg, 2.5\u2009mg\/kg MeHg, 0.5\u2009mg\/kg MeHg\u2009+\u200920\u2009mg\/kg fisetin, and 2.5\u2009mg\/kg MeHg\u2009+\u200920\u2009mg\/kg fisetin. The pregnant females were orally dosed on GDs 7, 12, and 17. The study evaluated maternal water intake along with renal function indicators, including serum creatinine and blood urea nitrogen (BUN) levels. Offspring (litters) were assessed for body weight on postnatal day (PND) 7 and PND 21. Additionally, the expression levels of fibrotic markers\u2014MMP\u20109, SMAD\u20103, and alpha\u2010SMA\u2014were analyzed using quantitative PCR and Western blot techniques in kidney tissue. Moreover, histopathological analysis of kidney tissues was also conducted to examine potential structural alterations. Results indicated that both the MeHg exposures led to significant nephrotoxicity, evidenced by elevated creatinine and BUN levels, upregulation of fibrotic genes, and pronounced structural damage in the kidneys. However, fisetin administration mitigated these adverse effects, normalizing renal function biomarkers, downregulating fibrotic gene expression, and preserving kidney structure. Our study, therefore, underscores the potential of fisetin as a protective agent against MeHg\u2010induced renal damage during prenatal development, offering insights into its therapeutic role in safeguarding fetal health against environmental toxins.<\/jats:p>","DOI":"10.1002\/jat.4979","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T06:26:07Z","timestamp":1762496767000},"page":"1629-1640","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Therapeutic Effect of Fisetin in Mitigating Methylmercury\u2010Induced Nephrotoxicity During Prenatal Development"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9276-9324","authenticated-orcid":false,"given":"Sameen","family":"Shafi","sequence":"first","affiliation":[{"name":"Department of Zoology Mahatma Gandhi Central University  Motihari Bihar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hafizurrahman","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Zoology Maharani Janki Kunwar College  Bettiah Bihar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad\u00a0Kaleem","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Biochemistry King George Medical University  Lucknow UP India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Preeti","family":"Bajpai","sequence":"additional","affiliation":[{"name":"Department of Zoology Mahatma Gandhi Central University  Motihari Bihar 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