{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T09:08:27Z","timestamp":1780304907993,"version":"3.54.0"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T00:00:00Z","timestamp":1765152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T00:00:00Z","timestamp":1765152000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Di(2\u2010ethylhexyl) phthalate (DEHP), a widely used plasticizer, crosses the placental barrier and disrupts fetal development, though its precise mechanisms remain poorly defined. To assess its placental effects, pregnant mice were treated daily with 40 \u03bcg\/kg DEHP, and placental alterations were systematically evaluated through histopathological examination, Western blot analysis, and whole\u2010transcriptome sequencing. DEHP exposure reduced the total placental area, disrupted the decidual and spongiotrophoblast layers, and disorganized vascular structures. Concurrently, protein levels of Bax, p53, and Caspase\u20103 were significantly elevated, while Bcl\u20102 expression decreased, indicating mitochondrial apoptosis mediated by the PI3K\/Akt\u2013p53 pathway. Transcriptomic profiling further revealed widespread dysregulation of mRNAs and noncoding RNAs, enriched in processes involving the Notch signaling pathway, cholesterol metabolism, hormone response, and oxygen transport. Construction of a ceRNA network suggested that miR\u20106538 may contribute to placental dysfunction by regulating Tmcc2 and Susd2. These findings elucidate a novel mechanism through which DEHP impairs placental structure and function via apoptotic signaling and RNA regulatory networks, offering important insights into its reproductive toxicity.<\/jats:p>","DOI":"10.1002\/jat.70016","type":"journal-article","created":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T00:52:00Z","timestamp":1765241520000},"page":"2309-2319","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Di(2\u2010ethylhexyl) Phthalate Induces Placental Cellular Apoptosis via the PI3K\/Akt\u2010p53 Pathway and Functional Disruption via a ceRNA Network"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0187-0021","authenticated-orcid":false,"given":"Yang","family":"Dong","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Qingdao Agricultural University  Qingdao 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