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Dietary exposure to these compounds may pose a health risk because their free forms exhibit toxic effects that have been linked to renal cancer and other adverse outcomes. Considering that the role of microRNAs (miRNAs) in the toxicity of these contaminants remains poorly understood in the literature, the aim of this study was to investigate changes in miRNA expression linked to renal carcinogenesis using RT\u2010PCR and to 3\u2010MCPD and glycidol. Tumor (786\u20100) and non\u2010tumor (HK\u20102 and HEK\u2010293) renal cell lines were exposed to contaminants for 40 and 80\u2009days at low and high concentrations, respectively. Our results demonstrated that glycidol was more toxic than 3\u2010MCPD in renal cell lines, with IC50 values up to 200\u2009mM for 3\u2010MCPD compared to 7.19\u2009mM for glycidol. Furthermore, we observed up to an 11\u2010fold upregulation of miRNAs associated with renal carcinogenesis and a downregulation of tumor suppressor miRNAs. Our data provide new insights that may be relevant for future studies investigating the mechanisms of renal carcinogenesis induced by 3\u2010MCPD and glycidol.<\/jats:p>","DOI":"10.1002\/jat.70027","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T04:15:21Z","timestamp":1766031321000},"page":"2521-2527","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["MicroRNA Expression During Long\u2010Term Treatment With 3\u2010MCPD and Glycidol In\u00a0Vitro"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3222-8950","authenticated-orcid":false,"given":"Renan\u00a0G.","family":"Tivanello","sequence":"first","affiliation":[{"name":"Faculty of Food Engineering University of Campinas (UNICAMP)  Campinas SP 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