{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T02:14:35Z","timestamp":1779243275987,"version":"3.51.4"},"reference-count":59,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The transport of pharmaceutical compounds into aquatic ecosystems poses a significant environmental threat, particularly due to the presence of drugs that cannot be completely removed during wastewater treatment processes. Diclofenac (DCF), one of the most widely used nonsteroidal anti\u2010inflammatory drugs worldwide, is among the pharmaceuticals frequently detected in aquatic environments due to its high consumption levels and persistence in the environment. It is known that this compound causes neurotoxicity, behavioral disorders, and physiological stress responses in aquatic organisms even at low concentrations. This study aimed to determine the effects of diclofenac exposure on oxidative stress, circadian rhythm, and behavioral parameters in zebrafish larvae. For this purpose, zebrafish embryos and early\u2010stage larvae were exposed to DCF at concentrations of 0.5, 2.5, and 12.5\u2009\u03bcg\/L for 120\u2009h. Subsequently, to investigate the effect of DCF on oxidative stress, SOD, CAT, GPX, and AChE enzyme activities and gene expression levels were analyzed. To examine its effects on behavior and circadian rhythm, thigmotaxis and locomotor activity analyses were performed. Additionally, to determine the molecular\u2010level effects of behavioral changes, the expression levels of the\n                    <jats:italic>bdnf<\/jats:italic>\n                    ,\n                    <jats:italic>5ht4<\/jats:italic>\n                    ,\n                    <jats:italic>crhr<\/jats:italic>\n                    ,\n                    <jats:italic>bmal1<\/jats:italic>\n                    ,\n                    <jats:italic>per<\/jats:italic>\n                    , and\n                    <jats:italic>gnat2<\/jats:italic>\n                    genes were analyzed. Overall, our findings indicate that DCF affects behavioral activity, neurotransmitter metabolism, oxidative stress response, circadian rhythm, and retina\u2010related molecular regulators in zebrafish larvae in a multilevel manner. These results highlight the potential risks of pharmaceutical contaminants on neurodevelopmental processes in aquatic ecosystems and demonstrate that even environmental doses can produce complex responses in biological systems.\n                  <\/jats:p>","DOI":"10.1002\/jat.70089","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T03:16:07Z","timestamp":1770261367000},"page":"1389-1398","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dose\u2010Dependent Neurobehavioral and Molecular Responses to Diclofenac in Zebrafish Larvae"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4414-9873","authenticated-orcid":false,"given":"Ekrem","family":"Sulukan","sequence":"first","affiliation":[{"name":"Department of Basic Sciences, Faculty of Fisheries Atat\u00fcrk University  Erzurum 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