{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T17:53:59Z","timestamp":1785779639720,"version":"3.56.0"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Zinc oxide nanoparticles (ZnO\u2010NPs) are being progressively employed in various industries, including cosmetics, textiles, and sun protection products. Due to their widespread application, concerns have emerged about their potential health hazards, especially through environmental exposure. This study was conducted to assess the acute and subacute toxicological impact of orally administered ZnO\u2010NPs (30\u2009\u00b1\u20095\u2009nm) on the pancreas of adult male albino rats. In this study, the size of ZnO\u2010NPs was determined by using transmission electron microscopy (TEM) as provided by the company. Eighty male albino rats (2.5\u20133\u2009months old) were allocated into two equal groups to evaluate acute (1\u2009day) and subacute (28\u2009days) ZnO\u2010NP toxicity (40 rats each). Each was further divided into control (negative and positive), low\u2010dose (175\u2009mg\/kg), and high\u2010dose (350\u2009mg\/kg) subgroups. Blood was collected via cardiac puncture under anesthesia for biochemical analysis, and pancreatic tissues were examined histopathologically and immunohistochemically. Both acute and subacute ZnO\u2010NP exposure caused a significant rise in blood glucose and serum Zn. Insulin levels increased acutely but decreased with subacute exposure. The subacute group showed a significant increase in oxidative stress markers indicated by elevated malondialdehyde (MDA) compared to controls. Histological examination revealed pancreatic degeneration, whereas increased inducible nitric oxide synthase (iNOS) immunopositivity indicated an inflammatory response, suggesting the involvement of oxidative stress in both groups. Oral exposure to toxic doses of ZnO\u2010NPs caused oxidative stress and structural damage in pancreatic cells, affecting insulin and glucose levels. These findings highlight the potential health risks of ZnO\u2010NP exposure, particularly with prolonged use.<\/jats:p>","DOI":"10.1002\/jat.70109","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:15:02Z","timestamp":1771373702000},"page":"3192-3208","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Zinc Oxide Nanoparticles' Acute and Subacute Oral Toxic Effects on Pancreas in Adult Male Albino Rats"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3115-9516","authenticated-orcid":false,"given":"Mennat\u2010Allah\u00a0Ahmed","family":"El\u00a0Refaey","sequence":"first","affiliation":[{"name":"Forensic Medicine and Clinical Toxicology, Faculty of Medicine Tanta University  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doaa\u00a0Mohamed","family":"El\u2010Gharbawy","sequence":"additional","affiliation":[{"name":"Clinical Toxicology, Faculty of Medicine Tanta University  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reda\u00a0Hassan","family":"ElBakary","sequence":"additional","affiliation":[{"name":"Histology and Cell Biology, Faculty of Medicine Tanta University  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merfat\u00a0Mondy","family":"Oreby","sequence":"additional","affiliation":[{"name":"Clinical Toxicology, Faculty of Medicine Tanta University  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdy\u00a0Mohamed","family":"Ashmawy","sequence":"additional","affiliation":[{"name":"Forensic Medicine and Clinical Toxicology, Faculty of Medicine Tanta University  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1638"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.21608\/ejfsat.2018.4194.1017"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.43.4.564"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.21608\/smj.2022.173745.1354"},{"key":"e_1_2_11_6_1","volume-title":"Theory and Practice of Histological Techniques","author":"Bancroft J. 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