{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:27:34Z","timestamp":1788276454728,"version":"build-2803163510"},"reference-count":59,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Gold has been used for centuries in both ornamental and medicinal contexts. More recently, gold compounds, containing ions or nanoparticles, have attracted attention for their anti\u2010inflammatory, anticancer, and diagnostic applications. Therefore, concerns about systemic toxicity and biodistribution have prompted investigations into the biological effects of gold in animal models and humans. In the present study, we explored the impacts of a gold solution, used in chrysotherapy, on the reproductive function of male Wistar rats. Seven chronic intraperitoneal injections of sodium 3\u2010aurothio\u20102\u2010hydroxypropane\u20101\u2010sulfonate at 30% of gold (allochrysine) were administered to adult male Wistar rats over a period of 2\u2009weeks. The control rats received 0.9% NaCl solution. Our results showed that gold has no notable effect on the absolute weight of reproductive organs (testicles, epididymides, seminal vesicles, and the two caudae of the epididymides) and of nonreproductive organs (liver and brain) excepting the kidneys. The assessment of the fertility parameters showed a significant decline in the motility and viability of sperm cells. A slight reduction in the sperm density, and many malformations affecting the spermatozoa were detected, and the testosterone levels in rats' blood were also significantly diminished. Our study showed that administration of gold solution reduced sperm parameters and was a disruptive agent for the endocrine function of testicles. The oxidative stress generation, attributed to this heavy metal, led to a significant hypertrophy of the kidneys probably after inflammation. Thus, gold existing in different medical treatments could be classified as an endocrine disruptor and could be a detrimental factor of male fertility.<\/jats:p>","DOI":"10.1002\/jat.70129","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:34:50Z","timestamp":1772991290000},"page":"3403-3414","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Assessment of Gold\u2010Mediated Male Reproductive Dysfunction in Male WISTAR Rats Through Sperm Analysis and Testosterone Measurement"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4623-231X","authenticated-orcid":false,"given":"Samira","family":"Maghraoui","sequence":"first","affiliation":[{"name":"Laboratory of Physiology Faculty of Medicine of Tunis (University of Tunis El Manar)  Tunis Tunisia"},{"name":"Laboratory of Medical Biology Abderahmen Mami Hospital of Pneumology\u2010Phtisiology  Ariana Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0898-7626","authenticated-orcid":false,"given":"Adrian","family":"Florea","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology Faculty of Medicine, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy  Cluj\u2010Napoca Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7280-1382","authenticated-orcid":false,"given":"Leila","family":"Tekaya","sequence":"additional","affiliation":[{"name":"Laboratory of Physiology Faculty of Medicine of Tunis (University of Tunis El Manar)  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,26]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43188\u2010023\u201000203\u20102"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970\u20101591.78437"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011\u2010017\u20101135\u20107"},{"key":"e_1_2_12_5_1","article-title":"Gold Deposits Within Lysosomes of Placental Cells: Ultrastructural and Quantitative Study","volume":"3","author":"Badri N.","year":"2020","journal-title":"Annals of Obstetrics and Gynecology"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/NNANO.2010.153"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0NA00472C"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13280\u2010023\u201001831\u20106"},{"key":"e_1_2_12_9_1","first-page":"348","article-title":"The Effect of Gold Nanoparticles on Luteinizing Hormone, Follicle\u2010Stimulating Hormone, Testosterone, and Testis in Male Rats","volume":"26","author":"Behnammorshedi M.","year":"2015","journal-title":"Biomedical Research"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.2c00727"},{"key":"e_1_2_12_11_1","first-page":"395","article-title":"The Role of Lysosomes in the Selective Concentration of Mineral Elements: A Microanalytical Study","volume":"42","author":"Berry J. 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