{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:27:02Z","timestamp":1788276422863,"version":"build-2803163510"},"reference-count":57,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Liver fibrosis represents an abnormal wound\u2010healing response to chronic hepatic injury. It is characterized by the excessive production and deposition of extracellular matrix components, ultimately resulting in the formation of a fibrotic scar. Visnagin, a phytochemical, has antioxidants and anti\u2010inflammatory activity and modulates apoptosis; however, the role of visnagin in liver fibrosis has not been previously explored. Herein, we have induced liver fibrosis using thioacetamide (TAA; 200\u2009mg\/kg) through the intraperitoneal\n                    <jats:italic>(i.p.)<\/jats:italic>\n                    route every third day for 8\u2009weeks in Sprague Dawley rats. Visnagin (5 and 10\u2009mg\/kg) co\u2010treatment was given via the\n                    <jats:italic>i.p<\/jats:italic>\n                    . route every day for 8\u2009weeks. At the end of the visnagin intervention, various biochemical, oxidative stress parameters, histopathological assessments, and quantitative reverse\u2010transcription polymerase chain reaction (qRT\u2010PCR) analyses were performed. Visnagin administration resulted in a significant decrease in the liver\u2010to\u2010body weight ratio and liver injury markers, including alanine transaminase, aspartate aminotransferase, and alkaline phosphatase. Additionally, visnagin attenuated TAA\u2010induced oxidative stress by restoring levels of malondialdehyde, nitrite, and superoxide dismutase. Histopathological assessments confirmed the hepatoprotective effects of visnagin against TAA\u2010induced liver injury, as evidenced by a reduction in hepatocyte damage and collagen deposition. Further, at a molecular level, visnagin treatment reduces the expression of caspase\u20103 and genes related to inflammation (TLR4, TNF\u2010\u03b1, IL\u20106, NLRP3) and fibrosis (\u03b1\u2010SMA, Col1A1, TGF\u2010\u03b2, MMP9, and TIMP1) in TAA\u2010treated rats.\n                  <\/jats:p>","DOI":"10.1002\/jat.70218","type":"journal-article","created":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:39:57Z","timestamp":1776904797000},"page":"3573-3584","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Visnagin Attenuates Thioacetamide\u2010Induced Liver Injury and Fibrosis Through the Modulation of TLR4\u2010Mediated Inflammation and Downregulation of TGF\u2010\u03b2\/\u03b1\u2010SMA\u2010Driven Fibrogenesis"],"prefix":"10.1002","volume":"46","author":[{"given":"Yogender","family":"Goswami","sequence":"first","affiliation":[{"name":"Department of Pharmacology Central University of Punjab  Bathinda Punjab 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