{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:27:21Z","timestamp":1788276441733,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T00:00:00Z","timestamp":1777766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T00:00:00Z","timestamp":1777766400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Acrolein, a major toxicant in fire and industrial smoke, induces fatal acute lung injury; however, its underlying mechanism remains undefined. This study aimed to elucidate this mechanism. Thirty\u2010two SD rats were divided into four groups and exposed to acrolein via tracheal instillation at doses of 0, 0.1, 1, and 10\u2009mg\/kg, respectively. Lung function and histopathology were assessed by invasive plethysmography and H&amp;E staining; alveolar\u2010capillary membrane permeability, tight junction ultrastructure, and tight junction protein levels were assessed by Evans blue extravasation, transmission electron microscopy, and Western blot, respectively. Acrolein exposure triggered severe, dose\u2010dependent impairment of pulmonary function, with significantly elevated respiratory system resistance and tissue elastance, alongside reduced inspiratory capacity and quasi\u2010static compliance. Histological examination revealed progressive alveolar architecture disruption, inflammatory infiltration, and intra\u2010alveolar hemorrhage across all dose groups. Evans blue assay confirmed dose\u2010related increases in pulmonary barrier permeability. Ultrastructural analysis showed pronounced disintegration of tight junctions between endothelial and epithelial cells, correlating with acrolein concentration. Concordantly, Western blot revealed dose\u2010dependent downregulation of claudin\u201018, occludin, and ZO\u20101. Direct disruption of alveolar tight junctions by acrolein elucidates its mechanism of inhalation toxicity and identifies this process as a potential target for barrier\u2010stabilizing interventions.<\/jats:p>","DOI":"10.1002\/jat.70235","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:01:17Z","timestamp":1777874477000},"page":"3622-3631","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deciphering the Pathogenic Cascade of Acrolein\u2010Induced Lung Injury: From Molecular Insult to Organ Failure"],"prefix":"10.1002","volume":"46","author":[{"given":"Zhihua","family":"Zhu","sequence":"first","affiliation":[{"name":"School of Public Health Baotou Medical College  Baotou China"},{"name":"Chinese PLA Center of Disease Control and Prevention  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Chinese PLA Center of Disease Control and Prevention  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8321-9902","authenticated-orcid":false,"given":"Hongjuan","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Aviation General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixi","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Public Health China Medical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4392-0889","authenticated-orcid":false,"given":"Qiang","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health Baotou Medical College  Baotou China"},{"name":"Chinese PLA Center of Disease Control and Prevention  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-19539-0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2025.154158"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbc.2023.100019"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-018-5209-6"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201100279"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0291"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/fam.946"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods11203203"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfu165"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.151.5.7735608"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2025.1598991"},{"issue":"6","key":"e_1_2_10_13_1","first-page":"417","article-title":"A Rare Cause of the Pulmonary\u2010Renal Syndrome: A Case of Atypical Haemolytic\u2010Uraemic Syndrome Complicated by Pulmonary Haemorrhage","volume":"1","author":"Derebail V. 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