{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:06:03Z","timestamp":1780445163716,"version":"3.54.1"},"reference-count":72,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFD2401803"],"award-info":[{"award-number":["2024YFD2401803"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Enrofloxacin (ENR) is a commonly identified veterinary pharmaceutical in global aquaculture products, and short\u2010term therapeutic applications of ENR at recommended doses may exert adverse effects on farmed fish. The present investigation evaluated the toxicological impacts of ENR on intestinal detoxification capacity, immune response, and microbial community dynamics of commercially significant\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Acanthopagrus schlegelii<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Short\u2010term treatment of ENR at the clinically recommended dose (5\u2009mg\/L) showed little modulation on expression levels of key detoxification enzymes, while 10\u2009mg\/L ENR (over\u2010therapeutic concentration) induced significantly elevated Phase I\/II biotransformation enzymes in treated fish. Interestingly, 5\u2009mg\/L ENR (decontaminated for 72\u2009h) triggered immunostimulation effects via significantly promoting inflammatory cytokines and lysozyme gene expression. In addition, persistent suppression of the diversity and richness of gut microbial communities was observed following immersion in 5 and 10\u2009mg\/L ENR. ENR exposure triggered restructuring of intestinal microbial composition of black seabream, characterized by a reduction in beneficial microbiota and an expansion of opportunistic and antibiotic\u2010resistant bacteria, which may contribute to the observed immune perturbation and long\u2010term health risks. In summary, our findings raise concerns about the safe use of ENR in aquaculture, suggesting that current practices may pose long\u2010term health risks to marine farmed fish.\n                  <\/jats:p>","DOI":"10.1002\/jat.70260","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T23:44:07Z","timestamp":1780443847000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic\u2010Dose Enrofloxacin Immersion Induces Intestinal Immunostimulation and Microbiota Dysbiosis in Juvenile Black Seabream\n                    <scp>\n                      <i>Acanthopagrus schlegelii<\/i>\n                    <\/scp>"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1101-5131","authenticated-orcid":false,"given":"Linbao","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Marine Ranching, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Ranching, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefeng","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Fisheries Guangdong Ocean University  Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Ranching, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haigang","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Ranching, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,2]]},"reference":[{"key":"e_1_2_9_2_1","article-title":"Unraveling Antibiotic Resistance in Achromobacter mucicolens IA Strain: Genomic Insights, Structural Analysis, and Prospects for Targeted Therapeutics","volume":"12","author":"Al\u2010Asadi S. A.","year":"2024","journal-title":"Microbiology Spectrum"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.envpol.2013.07.046","article-title":"Biphasic Dose Responses in Biology, Toxicology and Medicine: Accounting for Their Generalizability and Quantitative features","volume":"182","author":"Calabrese E. J.","year":"2013","journal-title":"Environmental Pollution"},{"key":"e_1_2_9_4_1","article-title":"Exposure to Enrofloxacin and Depuration: Endocrine Disrupting Effect in Juvenile Grass Carp (Ctenopharyngodon idella)","volume":"257","author":"Cao X. Q.","year":"2022","journal-title":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"},{"key":"e_1_2_9_5_1","article-title":"Effects of Enrofloxacin's Exposure on the Gut Microbiota of Tilapia Fish (Oreochromis niloticus)","volume":"46","author":"Chen J.","year":"2023","journal-title":"Comparative Biochemistry and Physiology Part D: Genomics and Proteomics"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: An Ultra\u2010Fast All\u2010in\u2010One FASTQ Preprocessor","volume":"34","author":"Chen S.","year":"2018","journal-title":"Bioinformatics"},{"key":"e_1_2_9_7_1","first-page":"34","article-title":"Effect on Antioxidant Enzymes Activity of Juvenile Trachinotus ovatus by Enrofloxacin","volume":"26","author":"Chen X.","year":"2019","journal-title":"Journal of Tian jin Agricultural University"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/jfb.70018","article-title":"Effects of Enrofloxacin and Povidone\u2010Iodine on Immunity, the Intestinal Microbiome and Transcriptome of Juvenile Grass Carp (Ctenopharyngodon idella)","volume":"107","author":"Chen Z.","year":"2025","journal-title":"Journal of Fish Biology"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","first-page":"S53","DOI":"10.1016\/j.jaci.2009.07.008","article-title":"Immunologic Messenger Molecules: Cytokines, Interferons, and Chemokines","volume":"125","author":"Commins S. P.","year":"2010","journal-title":"Journal of Allergy and Clinical Immunology"},{"key":"e_1_2_9_10_1","first-page":"127","article-title":"Simultaneous Determination of Seventeen Quinolones in Aquaculture Seawater Using Solid\u2010Phase Extraction and Liquid Chromatography Tandem Mass Spectrometry","volume":"38","author":"Dong X.","year":"2017","journal-title":"Progress In Fishery Sciences"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1038\/nmeth.2604","article-title":"UPARSE: Highly Accurate OTU Sequences From Microbial Amplicon Reads","volume":"10","author":"Edgar R. C.","year":"2013","journal-title":"Nature Methods"},{"key":"e_1_2_9_12_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.egg.2019.100037","article-title":"Phylogenetic Characterization of Eukaryotic and Prokaryotic Gut Flora of Nile Tilapia, Oreochromis niloticus, Along Niches of Lake Nasser, Egypt, Based on rRNA Gene High\u2010Throughput Sequences","volume":"11","author":"Elsaied H. E.","year":"2019","journal-title":"Ecological Genetics and Genomics"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.marpolbul.2023.115923","article-title":"The Enrofloxacin Pollution Control From Fish to Environment","volume":"199","author":"Feng X.","year":"2024","journal-title":"Marine Pollution Bulletin"},{"key":"e_1_2_9_14_1","first-page":"62","article-title":"Effects of Enrofloxacin on the Physiology and Biochemistry of Amphiprion ocellaris","volume":"26","author":"Gao H. W.","year":"2019","journal-title":"Journal of Tian jin Agricultural University"},{"key":"e_1_2_9_15_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodcont.2021.108780","article-title":"Residues of Veterinary Drugs in Fish and Fish Products: An Analysis of RASFF Data over the Last 20 Years","volume":"135","author":"Guardone L.","year":"2022","journal-title":"Food Control"},{"key":"e_1_2_9_16_1","doi-asserted-by":"crossref","first-page":"781","DOI":"10.3945\/jn.116.245506","article-title":"The Growth\u2010Promoting Effect of Dietary Nucleotides in Fish Is Associated With an Intestinal Microbiota\u2010Mediated Reduction in Energy Expenditure","volume":"147","author":"Guo X.","year":"2017","journal-title":"Journal of Nutrition"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2020.105551","article-title":"Distribution, Combined Pollution and Risk Assessment of Antibiotics in Typical Marine Aquaculture Farms Surrounding the Yellow Sea, North China","volume":"138","author":"Han Q. F.","year":"2020","journal-title":"Environment International"},{"key":"e_1_2_9_18_1","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.fsi.2022.10.050","article-title":"Traditional Chinese Herb Formulas in Diet Enhance the Non\u2010Specific Immune Responses of Yellow Catfish (Pelteobagrus fulvidraco) and Resistance Against Aeromonas hydrophila","volume":"131","author":"Hou T.","year":"2022","journal-title":"Fish & Shellfish Immunology"},{"key":"e_1_2_9_19_1","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/j.1365-2885.2011.01318.x","article-title":"Effects of Enrofloxacin on Cytochromes P4501A and P4503A in Carassius auratus gibelio (Crucian Carp)","volume":"35","author":"Hu X.","year":"2012","journal-title":"Journal of Veterinary Pharmacology and Therapeutics"},{"key":"e_1_2_9_20_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.foodcont.2017.09.019","article-title":"Occurrence of Veterinary Drug Residues in Farmed Fishery Products in South Korea","volume":"85","author":"Kang H. S.","year":"2018","journal-title":"Food Control"},{"key":"e_1_2_9_21_1","first-page":"105","article-title":"Effects of Triclosan on the Detoxification System in the Yellow Catfish (Pelteobagrus fulvidraco): Expressions of CYP and GST Genes and Corresponding Enzyme Activity in Phase I, II and Antioxidant System","volume":"166","author":"Ku P.","year":"2014","journal-title":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"},{"key":"e_1_2_9_22_1","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.it.2017.06.003","article-title":"Host Genetics and Gut Microbiome: Challenges and Perspectives","volume":"38","author":"Kurilshikov A.","year":"2017","journal-title":"Trends in Immunology"},{"key":"e_1_2_9_23_1","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1111\/jam.12475","article-title":"Characterization of the Gut Microbiota of Three Commercially Valuable Warmwater Fish Species","volume":"116","author":"Larsen A.","year":"2014","journal-title":"Journal of Applied Microbiology"},{"key":"e_1_2_9_24_1","first-page":"53","article-title":"Current Situation and Detection Methods of Enrofloxacin Residues in Aquatic Products in the Market","volume":"2","author":"Li L.","year":"2023","journal-title":"Farm Products Processing"},{"key":"e_1_2_9_25_1","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1111\/raq.12511","article-title":"A Global Analysis on the Systemic Effects of Antibiotics in Cultured Fish and Their Potential Human Health Risk: A Review","volume":"13","author":"Limbu S. M.","year":"2021","journal-title":"Reviews in Aquaculture"},{"key":"e_1_2_9_26_1","first-page":"96","article-title":"Effect of Enrofloxacin Treatment on Digestive Enzymes, Immune Enzymes and Antioxidant Enzymes in Trachinotus ovatus","volume":"46","author":"Lin S. Q.","year":"2019","journal-title":"Fisheries Science and Technology Information"},{"key":"e_1_2_9_27_1","first-page":"3977","article-title":"Effect of Plectropomus leopardus Antioxidant and Digestive Enzymes Activity to the Enrofloxacin Short Term Bath Administration","volume":"39","author":"Lin S. Q.","year":"2020","journal-title":"Genomics and Applied Biology"},{"key":"e_1_2_9_28_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2023.140751","article-title":"The Effects of Enrofloxacin Exposure on Responses to Oxidative Stress, Intestinal Structure and Intestinal Microbiome Community of Largemouth Bass (Micropterus salmoides)","volume":"348","author":"Liu C.","year":"2024","journal-title":"Chemosphere"},{"key":"e_1_2_9_29_1","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1007\/s00253-015-7039-6","article-title":"Denitrifying Sulfide Removal Process on High\u2010Salinity Wastewaters in the Presence of Halomonas sp","volume":"100","author":"Liu C.","year":"2016","journal-title":"Applied Microbiology and Biotechnology"},{"key":"e_1_2_9_30_1","article-title":"Short\u2010Term Exposure of Enrofloxacin Inhibits Synthesis of Central Estradiol through Secretoneurin a\/Aromatase B (cyp19a1b) Pathway in Crucian Carp","volume":"289","author":"Liu H.","year":"2025","journal-title":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"},{"key":"e_1_2_9_31_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2024.142573","article-title":"Dynamic Impacts of Short\u2010Term Bath Administration of Enrofloxacin on Juvenile Black Seabream Acanthopagrus schlegelii","volume":"361","author":"Liu Q.","year":"2024","journal-title":"Chemosphere"},{"key":"e_1_2_9_32_1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Realtime Quantitative PCR and the 2\u2010\u0394\u0394CT Method","volume":"25","author":"Livak K. J.","year":"2001","journal-title":"Methods"},{"key":"e_1_2_9_33_1","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.fsi.2004.09.006","article-title":"Innate Immunity of Fish (Overview)","volume":"20","author":"Magnad\u00f3ttir B.","year":"2006","journal-title":"Fish & Shellfish Immunology"},{"key":"e_1_2_9_34_1","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1093\/bioinformatics\/btr507","article-title":"FLASH: Fast Length Adjustment of Short Reads to Improve Genome Assemblies","volume":"27","author":"Mago T.","year":"2011","journal-title":"Bioinformatics"},{"key":"e_1_2_9_35_1","doi-asserted-by":"crossref","first-page":"5206","DOI":"10.1038\/s41467-020-18871-1","article-title":"Health and Disease Markers Correlate With Gut Microbiome Composition Across Thousands of People","volume":"11","author":"Manor O.","year":"2020","journal-title":"Nature Communications"},{"issue":"3","key":"e_1_2_9_36_1","first-page":"23","article-title":"Isolation and Characterization of Pseudomonas putida, a Causative Agent of Gastroenteritis in Black Sea Bream, Sparus macrocephalus","volume":"31","author":"Mao Z. J.","year":"2010","journal-title":"Progress in Fishery Sciences"},{"key":"e_1_2_9_37_1","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.mib.2018.07.003","article-title":"Low Diversity Gut Microbiota Dysbiosis: Drivers, Functional Implications and Recovery","volume":"44","author":"Michael K.","year":"2018","journal-title":"Current Opinion in Microbiology"},{"key":"e_1_2_9_38_1","first-page":"21","volume-title":"China Fishery Statistical Yearbook 2025","author":"Ministry of Agriculture and Rural Affairs, Bureau of Fisheries, National Fisheries Technology Extension Center","year":"2025"},{"key":"e_1_2_9_39_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2024.741216","article-title":"The Effect of Astragalus Polysaccharides on the Repair of Adverse Effects in Largemouth Bass (Micropterus salmoides) Under Enrofloxacin Stress","volume":"592","author":"Qi X.","year":"2024","journal-title":"Aquaculture"},{"key":"e_1_2_9_40_1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1186\/s40168-023-01574-2","article-title":"Vitamin B12 Produced by Cetobacterium somerae Improves Host Resistance Against Pathogen Infection Through Strengthening the Interactions Within Gut Microbiota","volume":"11","author":"Qi X.","year":"2023","journal-title":"Microbiome"},{"key":"e_1_2_9_41_1","first-page":"229","article-title":"Identification and Virulence Gene Analysis of a Pathogenic Pseudoalteromonas piscicida From Acanthopagrus schlegelii","volume":"43","author":"Qiao Y.","year":"2015","journal-title":"Jiangsu Agricultural Sciences"},{"key":"e_1_2_9_42_1","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.fsi.2005.01.010","article-title":"The Interferon System of Teleost Fish","volume":"20","author":"Robertsen B.","year":"2006","journal-title":"Fish & Shellfish Immunology"},{"key":"e_1_2_9_43_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2023.140315","article-title":"Disruption of Sex Steroid Hormones Biosynthesis by Short\u2010Term Enrofloxacin Antibiotic Exposure in Carassius auratus var. Pengze","volume":"344","author":"Ruan J.","year":"2023","journal-title":"Chemosphere"},{"key":"e_1_2_9_44_1","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1128\/CMR.14.4.778-809.2001","article-title":"Antiviral Actions of Interferons","volume":"14","author":"Samuel C. E.","year":"2001","journal-title":"Clinical Microbiology Reviews"},{"key":"e_1_2_9_45_1","doi-asserted-by":"crossref","first-page":"7537","DOI":"10.1128\/AEM.01541-09","article-title":"Introducing Mothur: Open\u2010Source, Platform\u2010Independent, Community\u2010Supported Software for Describing and Comparing Microbial Communities","volume":"75","author":"Schloss P. D.","year":"2009","journal-title":"Applied and Environmental Microbiology"},{"key":"e_1_2_9_46_1","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.28434","article-title":"The Who, Where and How of Fusobacteria and Colon Cancer","volume":"7","author":"Sears C. L.","year":"2018","journal-title":"eLife"},{"key":"e_1_2_9_47_1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.etap.2019.02.002","article-title":"Oxidative Stress Induced by Fluoroquinolone Enrofloxacin in Zebrafish (Danio rerio) Can Be Ameliorated After a Prolonged Exposure","volume":"67","author":"Sehonova P.","year":"2019","journal-title":"Environmental Toxicology and Pharmacology"},{"key":"e_1_2_9_48_1","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.tibtech.2015.06.011","article-title":"Proteobacteria: Microbial Signature of Dysbiosis in Gut Microbiota","volume":"33","author":"Shin N. R.","year":"2015","journal-title":"Trends in Biotechnology"},{"key":"e_1_2_9_49_1","first-page":"846","article-title":"Taxonomic Note: A Place for DNA\u2010DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology","volume":"44","author":"Stackebrandt E.","year":"1994","journal-title":"International Journal of Systematic Bacteriology"},{"key":"e_1_2_9_50_1","first-page":"9","article-title":"Effects of Lactic Acid on Drug\u2010Metabolizing Enzymes in Chinese Mitten Crab (Eriocheir sisnensis) After Oral Enrofloxacin","volume":"223","author":"Su H.","year":"2019","journal-title":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"},{"key":"e_1_2_9_51_1","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0044-8486(96)01319-1","article-title":"Antibacterial Abilities of Intestinal Bacteria in Freshwater Cultured Fish","volume":"145","author":"Sugita H.","year":"1996","journal-title":"Aquaculture"},{"key":"e_1_2_9_52_1","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.964283","article-title":"Occurrence and Human Health Risk Assessment of Antibiotics in Cultured Fish From 19 Provinces in China","volume":"12","author":"Tang Y.","year":"2022","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"e_1_2_9_53_1","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms10122373","article-title":"Characterization and Dynamics of the Gut Microbiota in Rice Fishes at Different Developmental Stages in Rice\u2010Fish Coculture Systems","volume":"10","author":"Tao L.","year":"2022","journal-title":"Microorganisms"},{"key":"e_1_2_9_54_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2022.160094","article-title":"Enrofloxacin Exposure Induces Anxiety\u2010Like Behavioral Responses in Zebrafish by Affecting the Microbiota\u2010Gut\u2010Brain Axis","volume":"858","author":"Tian D.","year":"2023","journal-title":"Science of the Total Environment"},{"key":"e_1_2_9_55_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2021.737119","article-title":"Population Pharmacokinetics\/Pharmacodynamics Modelling of Enrofloxacin for the Three Major Trout Pathogens Aeromonas salmonicida, Flavobacterium psychrophilum and Yersinia ruckeri","volume":"545","author":"Viel A.","year":"2021","journal-title":"Aquaculture"},{"key":"e_1_2_9_56_1","first-page":"33","article-title":"Preliminary Studies on Pathogen of Ascites Disease of Cultured Sparus macrocephalus","volume":"23","author":"Wang G. L.","year":"2003","journal-title":"Chinese Journal of Veterinary Science"},{"key":"e_1_2_9_57_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.marpolbul.2022.113777","article-title":"Occurrence of Quinolones in Cultured Fish From Shandong Province, China and Their Health Risk Assessment","volume":"180","author":"Wang X.","year":"2022","journal-title":"Marine Pollution Bulletin"},{"key":"e_1_2_9_58_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2021.118541","article-title":"Antibiotics in Mariculture Systems: A Review of Occurrence, Environmental Behavior, and Ecological Effects","volume":"293","author":"Wang X.","year":"2022","journal-title":"Environmental Pollution"},{"key":"e_1_2_9_59_1","first-page":"99","article-title":"Effects of Enrofloxacin on the Expression Analysis of CYP2 and Activity of APND in Litopenaeus vannamei","volume":"37","author":"Wang Y.","year":"2016","journal-title":"Progress in Fishery Sciences"},{"key":"e_1_2_9_60_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2025.137548","article-title":"Co\u2010Exposure to Enrofloxacin and Atrazine Enhances the Hepatotoxicity in Larimichthys crocea by Targeting the Hypothalamic\u2010Pituitary\u2010Thyroid and Gut\u2010Liver Axes","volume":"489","author":"Wang Y.","year":"2025","journal-title":"Journal of Hazardous Materials"},{"key":"e_1_2_9_61_1","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.scitotenv.2018.03.053","article-title":"Chronic Exposure to Low Concentrations of Lead Induces Metabolic Disorder and Dysbiosis of the Gut Microbiota in Mice","volume":"631","author":"Xia J.","year":"2018","journal-title":"Science of the Total Environment"},{"key":"e_1_2_9_62_1","article-title":"Effects of Cetobacterium somerae Fermentation Product on Gut and Liver Health of Common Carp (Cyprinus carpio) Fed Diet Supplemented with Ultra\u2010Micro Ground Mixed Plant Proteins","volume":"543","author":"Xie M.","year":"2021","journal-title":"Aquaculture"},{"key":"e_1_2_9_63_1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/BF02977789","article-title":"Induction of Phase I, II and III Drug Metabolism\/Transport by Xenobiotics","volume":"28","author":"Xu C.","year":"2005","journal-title":"Archives of Pharmacal Research"},{"key":"e_1_2_9_64_1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.aaf.2023.01.008","article-title":"Advances in Chemokines of Teleost Fish Species","volume":"9","author":"Xu H.","year":"2024","journal-title":"Aquaculture and Fisheries"},{"key":"e_1_2_9_65_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2021.737453","article-title":"Short\u2010Term Dietary Antibiotics Altered the Intestinal Microbiota and Improved the Lipid Metabolism in Hybrid Grouper Fed Medium and High\u2010Lipid Diets","volume":"547","author":"Xu J.","year":"2022","journal-title":"Aquaculture"},{"key":"e_1_2_9_66_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2021.737541","article-title":"Waterborne Enrofloxacin Exposure Activated Oxidative Stress and MAPK Pathway, Induced Apoptosis and Resulted in Immune Dysfunction in the Gills of Yellow Catfish Pelteobagrus fulvidraco","volume":"547","author":"Xu Y.","year":"2022","journal-title":"Aquaculture"},{"key":"e_1_2_9_67_1","first-page":"19","article-title":"Isolation of Edwardsiella tarda From Cultured Black Sea Bream (Sparus macrocephalus) and Its Identification","volume":"4","author":"Yan M. C.","year":"2010","journal-title":"Shandong Journal of Animal Science and Veterinary Medicine"},{"key":"e_1_2_9_68_1","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1111\/raq.12416","article-title":"Gut Microbiota Metagenomics in Aquaculture: Factors Influencing Gut Microbiome and Its Physiological Role in Fish","volume":"12","author":"Yukgehnaish K.","year":"2020","journal-title":"Reviews in Aquaculture"},{"key":"e_1_2_9_69_1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.ecoenv.2016.05.024","article-title":"Transcriptional Response of Four C1q Domain Containing Protein (C1qDC) Genes From Venerupis philippinarum Exposed to the Water Soluble Fraction of No.0 Diesel Oil","volume":"132","author":"Zhang L.","year":"2016","journal-title":"Ecotoxicology and Environmental Safety"},{"key":"e_1_2_9_70_1","doi-asserted-by":"crossref","DOI":"10.3390\/nu13103315","article-title":"Antibiotic\u2010Induced Gut Microbiota Dysbiosis Damages the Intestinal Barrier, Increasing Food Allergy in Adult Mice","volume":"13","author":"Zhang Q.","year":"2021","journal-title":"Nutrients"},{"key":"e_1_2_9_71_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2024.741062","article-title":"Evaluation of Enrofloxacin in the Chinese Soft\u2010Shelled Turtle (Pelodiscus sinensis) Based on the Biochemical, Histopathological and Intestinal Microbiota Responses","volume":"590","author":"Zhang Y.","year":"2024","journal-title":"Aquaculture"},{"key":"e_1_2_9_72_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2024.135611","article-title":"Neurotoxicity and Intestinal Microbiota Dysbiosis Induced by Per\u2010 and Polyfluoroalkyl Substances in Crucian Carp (Carassius auratus)","volume":"478","author":"Zhao Y.","year":"2024","journal-title":"Journal of Hazardous Materials"},{"key":"e_1_2_9_73_1","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1002\/vms3.267","article-title":"Could the Gut Microbiota Community in the Coral Trout Plectropomus leopardus (Lacep\u00e8de, 1802) Be Affected by Antibiotic Bath Administration?","volume":"6","author":"Zheng X.","year":"2020","journal-title":"Veterinary Medicine and Science"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.70260","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jat.70260","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.70260","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T23:44:17Z","timestamp":1780443857000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.70260"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,2]]},"references-count":72,"alternative-id":["10.1002\/jat.70260"],"URL":"https:\/\/doi.org\/10.1002\/jat.70260","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"value":"0260-437X","type":"print"},{"value":"1099-1263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,2]]},"assertion":[{"value":"2026-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"jat.70260"}}