{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T08:16:07Z","timestamp":1783498567020,"version":"3.55.0"},"reference-count":21,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Diethylpropion (DEP), a psychostimulant structurally related to amphetamine, is prescribed as an appetite suppressant but carries a potential risk of neurotoxicity and abuse. Here, we investigated the physiological and behavioral consequences of chronic DEP exposure during development in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    , combining in\u00a0vivo assays with\n                    <jats:italic>in silico<\/jats:italic>\n                    toxicity predictions. Age\u2010synchronized larvae were exposed to DEP (100\u2013500\u2009\u03bcM) for up to 72\u2009h, and survival, growth, reproduction, feeding, and neuromotor function were assessed. DEP reduced survival in a dose\u2010dependent manner, delayed larval development, decreased body size, and impaired reproductive capacity, including brood size reduction, shorter egg\u2010laying period, and increased intrauterine egg retention. Neuromotor activity, measured by head thrashes and body bends, as well as pharyngeal pumping rates, was significantly reduced. DEP also triggered oxidative stress, evidenced by upregulation of gst\u20104::GFP expression. In silico analysis predicted high neurotoxicity, oxidative stress potential, and endocrine disruption, with probable interactions in dopaminergic signaling and mitochondrial function. These findings indicate that developmental DEP exposure disrupts multiple physiological systems in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    , highlighting its potential to cause lasting neuromuscular and reproductive impairments.\n                  <\/jats:p>","DOI":"10.1002\/jat.70296","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T07:44:39Z","timestamp":1783496679000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Physiological and Behavioral Effects of Chronic Diethylpropion Exposure During Development in\n                    <scp>\n                      <i>Caenorhabditis elegans<\/i>\n                    <\/scp>\n                    : An In\u00a0Vivo and in Silico Toxicity Approach"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3252-4146","authenticated-orcid":false,"given":"L. R.","family":"Souza","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Psychology, Neuroscience and Behavior, Institute of Psychology Federal University of Rio Grande do Sul  Porto Alegre Brazil"},{"name":"Postgraduate Program in Biological Sciences: Neurosciences, Institute of Basic Health Sciences (ICBS) Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0562-9723","authenticated-orcid":false,"given":"G. O.","family":"Varriento","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropharmacology and Preclinical Toxicology, Institute of Basic Health Sciences Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7987-1359","authenticated-orcid":false,"given":"P.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropharmacology and Preclinical Toxicology, Institute of Basic Health Sciences Federal University of Rio Grande do Sul  Porto Alegre Brazil"},{"name":"Postgraduate Program in Biological Sciences: Pharmacology and Therapeutics, Institute of Basic Health Sciences (ICBS) Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5446-1695","authenticated-orcid":false,"given":"T. W.","family":"Viola","sequence":"additional","affiliation":[{"name":"School of Medicine, Brain Institute of Rio Grande do Sul, Developmental Cognitive Neuroscience Lab Pontifical Catholic University of Rio Grande do Sul (PUCRS)  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2450-2238","authenticated-orcid":false,"given":"R. M. M.","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Psychology, Neuroscience and Behavior, Institute of Psychology Federal University of Rio Grande do Sul  Porto Alegre Brazil"},{"name":"Postgraduate Program in Biological Sciences: Neurosciences, Institute of Basic Health Sciences (ICBS) Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3840-7343","authenticated-orcid":false,"given":"P. A.","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropharmacology and Preclinical Toxicology, Institute of Basic Health Sciences Federal University of Rio Grande do Sul  Porto Alegre Brazil"},{"name":"Postgraduate Program in Biological Sciences: Pharmacology and Therapeutics, Institute of Basic Health Sciences (ICBS) Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.brainres.2006.10.075","article-title":"Amphetamine\u2010Induced Locomotion, Behavioral Sensitization to Amphetamine, and Striatal D2 Receptor Function in Rats With High or Low Spontaneous Exploratory Activity: Differences in the Role of Locus Coeruleus","volume":"1131","author":"Alttoa A.","year":"2007","journal-title":"Brain Research"},{"issue":"1","key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0163-7258(03)00052-4","article-title":"Effects of Amphetamines on Mitochondrial Function: Role of Free Radicals and Oxidative Stress","volume":"99","author":"Brown J. 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