{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T13:04:38Z","timestamp":1784120678285,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cyclophosphamide is known to induce oxidative stress\u2013mediated reproductive injury. The present study investigated the prophylactic efficacy of ellagic acid and ellagic acid functionalized selenium nanoparticles against cyclophosphamide\u2010induced testicular injury in rats. Prophylactic treatment with ellagic acid (10, 20, 30, and 40\u2009mg\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    doses) and ellagic acid functionalized selenium nanoparticles (1, 2, 3, and 4\u2009mg\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    doses) was administered to Wistar rats prior to a single acute dose of cyclophosphamide (200\u2009mg\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ). Evaluation of biochemical parameters related to oxidative stress, antioxidant defence, lipid metabolism, and sperm functions along with histological alterations in the testis was carried out. Exposure to cyclophosphamide significantly elevated lipid peroxidation, depleted GSH, and suppressed enzymatic antioxidants, including superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glutathione S\u2010transferase in the testis, accompanied by increased total and esterified cholesterol in testicles and severe testicular degeneration. Prophylactic treatment with ellagic acid and ellagic acid functionalized selenium nanoparticles significantly reduced oxidative stress, enhanced activities of antioxidant enzymes, and improved testicular histoarchitecture and sperm indices. Notably, ellagic acid functionalized selenium nanoparticles offered protection comparably similar to ellagic acid, underscoring its potential as a promising nano\u2010formulation in mitigating cyclophosphamide\u2010induced testicular injury by modulating oxidative stress and biochemical alterations towards control. The close relationship between antioxidant imbalance and cyclophosphamide\u2010induced testicular injury was revealed by the findings. Prophylactic treatment with ellagic acid functionalized selenium nanoparticles was identified as a powerful nano\u2010enabled intervention supporting a selenium\u2010based nanotherapeutics\u2010based strategy for reproductive protection.\n                  <\/jats:p>","DOI":"10.1002\/jat.70351","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T12:09:19Z","timestamp":1784117359000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ellagic Acid and Ellagic Acid Functionalized Selenium Nanoparticles Ameliorate Cyclophosphamide\u2010Induced Testicular Injury by Modulating Oxidative Stress"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7047-1818","authenticated-orcid":false,"given":"Asim Amitabh","family":"Sahu","sequence":"first","affiliation":[{"name":"Toxicology and Pharmacology Laboratory, Department of Zoology Guru Ghasidas University  Koni\u2010Bilaspur Chhattisgarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satendra Kumar","family":"Nirala","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products, Department of Rural Technology and Social Development Guru Ghasidas University  Koni\u2010Bilaspur Chhattisgarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5213-2433","authenticated-orcid":false,"given":"Monika","family":"Bhadauria","sequence":"additional","affiliation":[{"name":"Toxicology and Pharmacology Laboratory, Department of Zoology Guru Ghasidas University  Koni\u2010Bilaspur Chhattisgarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00580\u2010021\u201003199\u2010z"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.70014"},{"key":"e_1_2_10_4_1","first-page":"121","volume-title":"Methods in Enzymology","author":"Aebi H.","year":"1984"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2014.05.011"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26020844"},{"issue":"6","key":"e_1_2_10_7_1","first-page":"512","article-title":"Regulation and Properties of Purified Glucose\u20106\u2010Phosphate Dehydrogenase From Rat Brain","volume":"33","author":"Askar M. 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