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In this study, the subacute toxicity of 9\u2010MF was evaluated in male and female Sprague\u2013Dawley rats following 14 consecutive days of intravenous administration. 9\u2010MF at 1\u20135\u2009mg\/kg did not induce significant alterations in body weight, food consumption, motor function, or anxiety\u2010like behavior. Sex\u2010dependent sensitivity to 9\u2010MF\u2010induced hepatotoxicity was observed. Furthermore, 9\u2010MF induced mild hepatic inflammation, red pulp hemorrhage in the spleen, inflammatory cell infiltration, and alveolar septal thickening in the lung, as well as mild neuronal degeneration and structural loosening in the dentate gyrus region of the brain. Integrated transcriptomic, proteomic, and metabolomic analyses further revealed that 5\u2009mg\/kg 9\u2010MF regulated 6\u2010pyruvoyltetrahydropterin synthase\u2010centered biopterin metabolism, arachidonate 15\u2010lipoxygenase\u2010centered linoleate metabolism, and the mTOR pathway in the brain, as well as\n                    <jats:italic>SMPD4<\/jats:italic>\n                    \/phytosphingosine\u2010centered sphingolipid metabolism,\n                    <jats:italic>PLA2G2A<\/jats:italic>\n                    \u2010centered arachidonic acid metabolism, and\n                    <jats:italic>GMPR<\/jats:italic>\n                    \u2010centered porphyrin metabolism in the blood, contributing to the observed abnormalities. These findings not only provided a toxicological basis for the further drug development of 9\u2010MF but also served as an example of a toxicological study integrating traditional assessments with multiomics strategies.\n                  <\/jats:p>","DOI":"10.1002\/jat.70364","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:16:35Z","timestamp":1785215795000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Subacute Toxicological Evaluation of 9\u2010Methylfascaplysin, a Marine\u2010Derived Neuroprotective Compound, via an Integrated Multiomics Approach"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-9526-6689","authenticated-orcid":false,"given":"Panchao","family":"Luo","sequence":"first","affiliation":[{"name":"The Affiliated Women and Children's Hospital of Ningbo University  Ningbo China"},{"name":"Translational Medicine Center of Pain, Emotion and 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