{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T04:30:33Z","timestamp":1786595433246,"version":"build-2736575974"},"reference-count":64,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Coumarins are phenolic compounds widely recognized for their diverse pharmacological activities and have attracted increasing attention as potential chemopreventive agents. In the present study, four known 3\u2010benzoylcoumarin derivatives [(\n                    <jats:bold>1<\/jats:bold>\n                    ), (\n                    <jats:bold>2<\/jats:bold>\n                    ), (\n                    <jats:bold>3<\/jats:bold>\n                    ), and (\n                    <jats:bold>4<\/jats:bold>\n                    )] were evaluated for their cytotoxic, mutagenic, and antigenotoxic effects in Chinese hamster lung fibroblasts (V79 cells). Cytotoxicity was initially assessed using the MTT assay to establish sub\u2010cytotoxic concentrations for subsequent analyses. Genotoxicity and chemopreventive activity were investigated using the cytokinesis\u2010block micronucleus (CBMN) assay following DNA damage induced by doxorubicin (DXR) or methyl methanesulfonate (MMS). All compounds exhibited concentration\u2010dependent cytotoxicity, with (\n                    <jats:bold>2<\/jats:bold>\n                    ) showing the greatest potency. In the CBMN assay, none of the compounds significantly altered the nuclear division index (NDI), indicating the absence of cytostatic effects. However, at higher concentrations, the derivatives increased micronucleus (MN) frequency, suggesting the induction of chromosomal damage. In the antigenotoxicity assay, differential protective effects were observed depending on the mutagen used. Cotreatment with DXR demonstrated that (\n                    <jats:bold>1<\/jats:bold>\n                    ) significantly reduced MN frequency at the highest tested concentration, whereas (\n                    <jats:bold>3<\/jats:bold>\n                    ) and (\n                    <jats:bold>4<\/jats:bold>\n                    ) significantly decreased DXR\u2010induced chromosomal damage. When MMS was used as the mutagenic agent, (\n                    <jats:bold>2<\/jats:bold>\n                    ) significantly reduced MN formation at lower concentrations. Taken together, these findings support a Janus\u2010like behavior of 3\u2010benzoylcoumarins and indicate dose\u2010dependent genotoxic and antigenotoxic properties and highlight their potential as modulators of DNA damage induced by different mutagenic mechanisms. Overall, these findings highlight the concentration\u2010dependent dual biological effects of 3\u2010benzoylcoumarins and support further investigations into their potential as chemopreventive agents.\n                  <\/jats:p>","DOI":"10.1002\/jat.70385","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T04:22:54Z","timestamp":1786594974000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dual Genotoxic and Antigenotoxic Effects of 3\u2010Benzoylcoumarins in Mammalian Cells"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4198-3475","authenticated-orcid":false,"given":"Leticia Helena","family":"Parreira","sequence":"first","affiliation":[{"name":"Universidade Federal de Alfenas Instituto de Ci\u00eancias da Natureza  Alfenas\/MG 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