{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T08:24:31Z","timestamp":1786955071378,"version":"build-2736575974"},"reference-count":90,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study evaluated both the acute and sublethal effects of pyriproxyfen in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Cyprinus carpio<\/jats:italic>\n                    <\/jats:styled-content>\n                    using an integrated multibiomarker approach. The 96\u2010h LC\n                    <jats:sub>50<\/jats:sub>\n                    value was calculated as 4.525\u2009mg\/L. Following 14\u2009days of sublethal exposure, pyriproxyfen caused clear, dose\u2010dependent changes across hematological, biochemical, genotoxic, and molecular parameters. Hemoglobin and hematocrit levels declined progressively with increasing concentration, pointing to the development of anemia\u2010like conditions. Biochemical analyses revealed a biphasic response pattern: Several parameters initially increased at lower concentrations but declined at higher doses, suggesting a shift from adaptive metabolic responses to hepatic impairment. DNA damage, assessed by the comet assay, increased significantly in all exposed groups, indicating genotoxic effects even at relatively low concentrations. At the molecular level, antioxidant and proinflammatory genes were upregulated at low to moderate doses, reflecting an early defense response. However, their expression was suppressed at higher concentrations, implying exhaustion of protective mechanisms. In contrast, HSP70 expression increased steadily with dose, highlighting the intensification of cellular stress and protein damage. Overall, the findings demonstrate that pyriproxyfen exposure triggers an initial adaptive response that progressively transitions into physiological dysfunction at higher concentrations, underscoring its potential ecological risk to aquatic organisms.\n                  <\/jats:p>","DOI":"10.1002\/jat.70399","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T07:38:12Z","timestamp":1786952292000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dose\u2010Dependent Toxicity of Pyriproxyfen in Common Carp (\n                    <scp>\n                      <i>Cyprinus carpio<\/i>\n                    <\/scp>\n                    ): Hematological, Biochemical, Genotoxic, and Molecular Responses"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6508-9817","authenticated-orcid":false,"given":"\u00dcmit","family":"Acar","sequence":"first","affiliation":[{"name":"Bayrami\u00e7 Vocational School \u00c7anakkale Onsekiz Mart University  \u00c7anakkale T\u00fcrkiye"},{"name":"Faculty of Fisheries Mu\u011fla S\u0131tk\u0131 Ko\u00e7man University  Mu\u011fla T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2591-0310","authenticated-orcid":false,"given":"\u00d6nder","family":"Y\u0131ld\u0131r\u0131m","sequence":"additional","affiliation":[{"name":"Faculty of Fisheries Mu\u011fla S\u0131tk\u0131 Ko\u00e7man University  Mu\u011fla T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0870-7049","authenticated-orcid":false,"given":"Rifat","family":"Tezel","sequence":"additional","affiliation":[{"name":"Faculty of Fisheries Mu\u011fla S\u0131tk\u0131 Ko\u00e7man University  Mu\u011fla T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2807-6096","authenticated-orcid":false,"given":"Yavuz","family":"Erden","sequence":"additional","affiliation":[{"name":"Faculty of Science Bart\u0131n University  Bart\u0131n T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2288-5084","authenticated-orcid":false,"given":"Sercan","family":"Yap\u0131c\u0131","sequence":"additional","affiliation":[{"name":"Faculty of Fisheries Mu\u011fla S\u0131tk\u0131 Ko\u00e7man University  Mu\u011fla T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2022.109494"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.4645"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123411"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695\u2010025\u201001467\u20101"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.5620\/eaht.2022012"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecolind.2013.09.016"},{"issue":"2","key":"e_1_2_8_8_1","first-page":"1","article-title":"Determination of LC50 and Investigation of Acute Toxicity Effects of Diazinon on Hematology and Serology Indices of Common Carp (Cyprinus carpio)","volume":"3","author":"Banaee M.","year":"2008","journal-title":"Journal of Marine Science and Technology Research"},{"key":"e_1_2_8_9_1","volume-title":"Climate Change\u2014Recent Observations","author":"Bashir M. 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