{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:31:25Z","timestamp":1787020285115,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100019733","name":"University of Health Sciences Lahore","doi-asserted-by":"publisher","award":["2023\/092"],"award-info":[{"award-number":["2023\/092"]}],"id":[{"id":"10.13039\/100019733","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Colorectal cancer (CRC) remains a major global health concern, accounting for 9.6% of all cancer cases and 9.3% of cancer\u2010related deaths. Natural compounds with anticancer properties have gained increasing interest due to their potential for reduced toxicity. This study investigated the cytotoxic, genotoxic, and apoptotic effects of naringenin\u2010oxime (NG\u2010Ox) on colorectal cancer (LoVo) and normal colon epithelial cells (CCD18\u2010Co). NG\u2010Ox was synthesized from NG, and its structure was verified by\n                    <jats:sup>1<\/jats:sup>\n                    H\u2010NMR spectroscopy. Cell viability was assessed using the MTT assay, apoptosis via acridine orange\/ethidium bromide staining, DNA damage by the Comet assay, and intracellular reactive oxygen species (iROS) levels through fluorometric analysis. Long\u2010term proliferative potential was evaluated using a colony formation assay, and principal component analysis (PCA) was employed to integrate the overall cellular responses. Both NG and NG\u2010Ox reduced LoVo cell viability in a dose\u2010dependent manner. NG showed greater selectivity for cancer cells, whereas NG\u2010Ox induced stronger DNA damage and apoptosis but also exhibited higher toxicity in normal cells. ROS induction was most prominent with 5\u2010FU, whereas NG and NG\u2010Ox produced moderate increases. PCA confirmed these findings, demonstrating an inverse correlation between cell viability and ROS, apoptosis, and DNA damage. NG was more closely associated with apoptosis and ROS responses, whereas NG\u2010Ox was more strongly associated with DNA damage. In conclusion, NG\u2010Ox showed strong DNA\u2010damaging and proapoptotic activity in CRC cells; however, its lack of selectivity compared with NG limits its immediate therapeutic potential.\n                  <\/jats:p>","DOI":"10.1002\/jat.70401","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:01:05Z","timestamp":1787018465000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Evaluation of Cytotoxic, Genotoxic, and Apoptotic Effects of Naringenin and Naringenin\u2010Oxime in Colorectal Cancer Cells"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2416-0773","authenticated-orcid":false,"given":"Kubra","family":"Bozali","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, Hamidiye School of Medicine University of Health Sciences Turkey  Istanbul T\u00fcrkiye"},{"name":"Department of Medical Biochemistry, Hamidiye Institute of Health Sciences University of Health Sciences Turkey  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4773-0161","authenticated-orcid":false,"given":"Sumeyye","family":"Koc","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Hamidiye School of Medicine University of Health Sciences Turkey  Istanbul T\u00fcrkiye"},{"name":"Department of Medical Biochemistry, Hamidiye Institute of Health Sciences University of Health Sciences Turkey  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9707-9111","authenticated-orcid":false,"given":"Beyza Nur","family":"Ozkan","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Hamidiye School of Medicine University of Health Sciences Turkey  Istanbul T\u00fcrkiye"},{"name":"Department of Medical Biochemistry, Hamidiye Institute of Health Sciences University of Health Sciences Turkey  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9278-8557","authenticated-orcid":false,"given":"Zeynep","family":"Ince","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Hamidiye School of Medicine University of Health Sciences Turkey  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3706-5193","authenticated-orcid":false,"given":"Hakan","family":"Beyaztas","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Hamidiye School of Medicine University of Health Sciences Turkey  Istanbul T\u00fcrkiye"},{"name":"Department of Medical Biochemistry, Hamidiye Institute of Health Sciences University of Health Sciences Turkey  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4351-1719","authenticated-orcid":false,"given":"Eray Metin","family":"Guler","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Hamidiye School of Medicine University of Health Sciences Turkey  Istanbul T\u00fcrkiye"},{"name":"Department of Medical Biochemistry, Haydarpasa Numune Health Application and Research Center, Hamidiye Faculty of Medicine University of Health Sciences Turkey  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.6061"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.4251\/WJGO.V8.I8.583"},{"issue":"1","key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1080\/01480545.2019.1683187","article-title":"Quercetin and Naringenin Abate Diethylnitrosamine\/Acetylaminofluorene\u2010Induced Hepatocarcinogenesis in Wistar Rats: The Roles of Oxidative Stress, Inflammation and Cell Apoptosis","volume":"45","author":"Ahmed O. 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