{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T03:14:51Z","timestamp":1788318891881,"version":"build-2803163510"},"reference-count":101,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The rapid advancement of nanotechnology has outpaced the development of adequate toxicity assessment methods, necessitating innovative analytical frameworks for evaluating emerging nano\u2010contaminants. The zebrafish (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Danio rerio<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) has emerged as a versatile vertebrate model that enables a comprehensive evaluation of molecular, physiological and developmental responses to nano\u2010contaminant exposure. This minireview synthesises recent advances in zebrafish\u2010based nanotoxicology, emphasising the integration of physicochemical characterisation with biological responses to improve predictive toxicity assessment. Current evidence identifies oxidative stress as one of the most extensively characterised molecular initiating events, activating interconnected pathways involving mitochondrial dysfunction, inflammation and apoptosis, ultimately driving organ\u2010specific and developmental toxicities. Combining physicochemical characterisation with multi\u2010omics approaches, high\u2010throughput phenotypic analyses and computational modelling enables a quantitative linkage between nanoparticle properties and toxicological outcomes within systems\u2010level and adverse outcome pathway (AOP)\u2010oriented frameworks. Together, these complementary approaches strengthen predictive toxicology while improving the mechanistic understanding of nanoparticle\u2010induced toxicity across diverse classes of emerging nano\u2010contaminants. Despite these advances, important challenges remain, including the lack of standardised protocols for nanoparticle characterisation and exposure, uncertainty in selecting appropriate exposure metrics and environmentally relevant experimental models. Addressing these limitations is essential for improving reproducibility, prediction accuracy and translational relevance. Overall, zebrafish\u2010based analytical toxicology provides a comprehensive framework for advancing predictive nanotoxicology thereby supporting safer\u2010by\u2010design nanomaterial development and evidence\u2010based risk assessment of emerging nano\u2010contaminants.\n                  <\/jats:p>","DOI":"10.1002\/jat.70425","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T02:41:37Z","timestamp":1788316897000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Zebrafish as an Integrated Analytical Framework for Mechanistic and Predictive Toxicity Assessment of Emerging Nano\u2010Contaminants"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9312-5288","authenticated-orcid":false,"given":"Seri Narti Edayu","family":"Sarchio","sequence":"first","affiliation":[{"name":"Department of Biomedical Science, Faculty of Medicine and Health Sciences Universiti Putra Malaysia  Serdang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8502-1566","authenticated-orcid":false,"given":"Suhaili","family":"Shamsi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia  Serdang Selangor Malaysia"},{"name":"Natural Medicine and Product Research Laboratory (NaturMeds), Institute of Bioscience Universiti Putra Malaysia  Serdang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"e_1_2_16_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2020.105646"},{"issue":"1","key":"e_1_2_16_3_1","first-page":"311","article-title":"Toxicity Assessment of Gallic Acid Loaded Graphene Oxide (GAGO) Nano\u2010Formulation in Zebrafish (Danio rerio) Embryos","volume":"28","author":"Abdul Ghafor A. 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