{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:53:27Z","timestamp":1791276807739,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Applied Toxicology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chronic pesticide exposure has been associated with oxidative stress, inflammation, and genomic instability, and microRNAs (miRNAs) may reflect regulatory responses occurring between exposure and adverse outcomes. This cross\u2010sectional biomonitoring study evaluated selected miRNAs as molecular response indicators associated with chronic pesticide exposure and genomic instability in 137 participants classified as putatively non\u2010exposed (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200934), indirectly exposed (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200941), or directly exposed agricultural workers (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200962). Buccal epithelial cells were analyzed for micronucleus frequency and expression of miR\u201021\u20105p, miR\u201034a\u20105p, miR\u2010146a\u20105p, miR\u2010155\u20105p, miR\u2010126\u20103p, and miR\u2010222\u20103p by RT\u2010qPCR. Hierarchical clustering, principal component analysis (PCA), logistic regression, ROC analysis, and repeated 10\u2010fold cross\u2010validation were performed. Directly exposed workers showed significantly increased expression of miR\u201021\u20105p, miR\u201034a\u20105p, miR\u2010146a\u20105p, miR\u2010155\u20105p, and miR\u2010222\u20103p, whereas miR\u2010126\u20103p was significantly reduced (all\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Micronucleus frequency increased progressively across the three groups (2.1\u2009\u00b1\u20090.4, 4.6\u2009\u00b1\u20090.7, and 7.8\u2009\u00b1\u20091.2 MN\/2000 cells;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). PCA explained 78.1% of the total variance and distinguished the exposure groups. The integrated six\u2010miRNA signature achieved an AUC of 0.92 (95% CI: 0.88\u20130.97) and an optimism\u2010corrected AUC of 0.89 after internal validation. These findings indicate that chronic pesticide exposure is associated with coordinated miRNA dysregulation and increased genomic instability. The six\u2010miRNA signature may complement established occupational biomonitoring approaches as an indicator of intermediate molecular responses, but it should not be interpreted as a direct measure of pesticide dose or clinical effect.\n                  <\/jats:p>","DOI":"10.1002\/jat.70450","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:30:21Z","timestamp":1791275421000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Analysis of Buccal\u2010Cell microRNAs Reveals Molecular Signatures Associated With Chronic Pesticide Exposure in Agricultural Workers"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4821-445X","authenticated-orcid":false,"given":"F\u00e1bio","family":"Morato de Oliveira","sequence":"first","affiliation":[{"name":"Laboratory of Human &amp; Medical Genetics Federal University of Jata\u00ed  Jata\u00ed Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Alves dos Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics and Molecular Biology University of Franca  Franca S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario Fernando","family":"Estorino Moreno","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics and Molecular Biology University of Franca  Franca S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fermino","family":"Lizarte Sanches Neto","sequence":"additional","affiliation":[{"name":"Faculty of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo \u2013 USP  Ribeir\u00e3o Preto S\u00e3o Paulo 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