{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T12:45:15Z","timestamp":1773665115701,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,7,22]],"date-time":"2003-07-22T00:00:00Z","timestamp":1058832000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"CONACYT;","award":["34394M."],"award-info":[{"award-number":["34394M."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Evidence shows that Na<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>\u2010ATPase from kidney, brain and liver is inhibited by nitric oxide (NO) and peroxynitrite anion (ONO<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>), but the mechanism is unknown. The aim of the present work was to study the inhibitory effect of NO and ONO<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> on erythrocyte Na<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>\u2010ATPase. Erythrocyte membranes were isolated from male Wistar rats by hypotonic washing. The membranes (free from haemoglobin) were incubated for Na<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>\u2010ATPase activity measurement at various concentrations of ATP in the presence or absence of 400 \u00b5M SNAP (an NO donor) or 100 \u00b5M SIN\u20101 (an ONO<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> donor). At these concentrations, SNAP and SIN\u20101 released about the same amount (100 \u00b5M) of NO or ONO<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>, respectively, as monitored by measuring NO<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> + NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>. Both SNAP and SIN\u20101 decreased <jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub> by ca. 75% but they did not decrease the apparent af\ufb01nity of the Na<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>\u2010ATPase for the substrate (a decrease of <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> was even observed after SNAP treatment). The pattern of this inhibition is compatible either with oxidation of SH groups directly involved in ATP binding but in a way that is not surmountable by increasing the substrate concentration (\u2018non\u2010competitive\u2019) or with oxidation of SH groups located outside the active site of the enzyme but important for the activity of the enzyme. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.922","type":"journal-article","created":{"date-parts":[[2003,7,23]],"date-time":"2003-07-23T06:13:29Z","timestamp":1058940809000},"page":"275-278","source":"Crossref","is-referenced-by-count":30,"title":["Insights into the mechanism of erythrocyte Na<sup>+<\/sup>\/K<sup>+<\/sup>\u2010ATPase inhibition by nitric oxide and peroxynitrite anion"],"prefix":"10.1002","volume":"23","author":[{"given":"Pablo","family":"Muriel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilberto","family":"Casta\u00f1eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica","family":"Ortega","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"No\u00ebl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,7,22]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(66)08014-5"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.4.1620"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(97)00053-4"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1002914201682"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90118-X"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)81150-X"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003737200"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/58.1.118"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1016\/S0021-9258(19)42943-8","article-title":"Effects of phospholipid acyl chain fluidity, phase transitions, and cholesterol on (Na+\/K+)\u2010stimulated adenosine triphosphatase","volume":"249","author":"Kimelberg HG","year":"1974","journal-title":"J. Biol. Chem"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00458a025"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.2550100409"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/niox.2000.0285"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/1099-1263(200011\/12)20:6<435::AID-JAT710>3.0.CO;2-O"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(84)90369-1"},{"key":"e_1_2_1_17_1","first-page":"1796","article-title":"Membrane adenosine triphosphate as a participant in the active transport of sodium and potassium in the human erythrocyte","volume":"235","author":"Post RL","year":"1960","journal-title":"J. Biochem. Chem"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)64313-7"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(76)90345-4"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)39314-7"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.68031312.x"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/S0022-3565(25)11228-7","article-title":"Lack of tolerance to a 24\u2010hour infusion of s\u2010nitroso n\u2010acetylpenicillamine (SNAP) in conscious rabbits","volume":"260","author":"Shaffer JF","year":"1992","journal-title":"J. Pharmacol. Exp. Ther"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.1166"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjat.922","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.922","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T13:02:58Z","timestamp":1760533378000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.922"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1002\/jat.922"],"URL":"https:\/\/doi.org\/10.1002\/jat.922","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"value":"0260-437X","type":"print"},{"value":"1099-1263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]}}}