{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T17:11:56Z","timestamp":1781716316636,"version":"3.54.5"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,9,8]],"date-time":"2003-09-08T00:00:00Z","timestamp":1062979200000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The single and combined effects of two abundant \ufb02avonoids, namely quercetin and genistein, were investigated according to their ability to inhibit the oxidation of methyl linolenate via Fenton's pathway. Antioxidative activity was determined by oxidizing methyl linolenate suspended in a buffer solution with either Fe<jats:sup>2+<\/jats:sup> (50 \u00b5M) or Cu<jats:sup>2+<\/jats:sup> (50 \u00b5M) and hydrogen peroxide (0.01 mM) without or with a \ufb02avonoid sample (10 or 20 \u00b5M). Lipid peroxidation products were measured by the thiobarbituric acid (TBA) assay and the amounts of thiobarbituric acid\u2010reactive substances (TBARS) were calculated from a calibration curve using 1,1,3,3\u2010tetraethoxypropane as the standard. Both quercetin and genistein at the 10 or 20 \u00b5M level decreased lipid peroxidation signi\ufb01cantly compared with their respective controls. Of the two \ufb02avonoids tested, quercetin had a more marked effect on inhibiting lipid peroxides. Peroxidation products for the control samples were higher for the Fe<jats:sup>2+<\/jats:sup>\u2010treated samples compared with the Cu<jats:sup>2+<\/jats:sup> samples. Combination of both \ufb02avonoids at the same dose levels continued to decrease lipid peroxidation, the effect being the same for both metal ions. The data suggest that the combined \ufb02avonoids offered better protection than the single treatments and this may be attributed to the better radical scavenging or increased chelating capabilities of the combined over the single treatments. The differences in peroxide levels for the single treatment of quercetin compared with the genistein\u2010treated samples may re\ufb02ect the structural differences between these compounds in combating oxidative stress. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.933","type":"journal-article","created":{"date-parts":[[2003,9,11]],"date-time":"2003-09-11T09:20:28Z","timestamp":1063272028000},"page":"363-369","source":"Crossref","is-referenced-by-count":28,"title":["Effect of quercetin and genistein on copper\u2010 and iron\u2010induced lipid peroxidation in methyl linolenate"],"prefix":"10.1002","volume":"23","author":[{"given":"William Y.","family":"Boadi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Iyere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel E.","family":"Adunyah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,9,8]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(94)90409-X"},{"key":"e_1_2_1_3_1","first-page":"10","volume-title":"Free Radicals in Biology","author":"Aust SD","year":"1982"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3301173"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(95)02014-4"},{"key":"e_1_2_1_6_1","first-page":"243","article-title":"Effects of flavonoids on induced non enzymic lipid peroxidation","volume":"213","author":"Das NP","year":"1994","journal-title":"Prog. 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