{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T14:52:52Z","timestamp":1772895172872,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,11,19]],"date-time":"2004-11-19T00:00:00Z","timestamp":1100822400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have reported previously that components of diesel exhaust particles (DEP) differently affect acute lung injury related to lipopolysaccharide (LPS) in mice. This study examined the effects of components of DEP on the lung expression of cyclooxygenase (COX)\u20101 and \u20102 in the presence or absence of LPS. ICR mice were divided into six experimental groups that received vehicle, LPS (2.5 mg kg<jats:sup>\u22121<\/jats:sup>), organic chemicals in DEP (DEP\u2010OC) extracted with dicloromethane (4 mg kg<jats:sup>\u22121<\/jats:sup>), residual carbonaceous nuclei after the extraction (washed DEP: 4 mg kg<jats:sup>\u22121<\/jats:sup>), DEP\u2010OC (4 mg kg<jats:sup>\u22121<\/jats:sup>) + LPS (2.5 mg kg<jats:sup>\u22121<\/jats:sup>) or washed DEP (4 mg kg<jats:sup>\u22121<\/jats:sup>) + LPS (2.5 mg kg<jats:sup>\u22121<\/jats:sup>) intratracheally. The expression of mRNA for both COXs in the lung was evaluated 4 h after the intratracheal administration. The magnitude of COX\u20101 mRNA expression was not altered in each group. The LPS treatment enhanced the COX\u20102 gene expression compared with vehicle treatment. Washed DEP combined with LPS further increased its expression compared with LPS alone. In contrast, combined treatment of DEP\u2010OC with LPS decreased COX\u20102 gene expression compared with LPS alone. These results suggest that the residual carbonaceous nuclei of DEP predominantly enhance lung expression of COX\u20102 rather than the extracted organic chemicals from DEP in the presence of LPS, which is concomitant with the magnitude of acute lung injury in our previous study. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.984","type":"journal-article","created":{"date-parts":[[2004,11,19]],"date-time":"2004-11-19T08:17:00Z","timestamp":1100852220000},"page":"415-418","source":"Crossref","is-referenced-by-count":10,"title":["Components of diesel exhaust particles differentially affect lung expression of cyclooxygenase\u20102 related to bacterial endotoxin"],"prefix":"10.1002","volume":"24","author":[{"given":"Ken\u2010ichiro","family":"Inoue","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirohisa","family":"Takano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rie","family":"Yanagisawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takamichi","family":"Ichinose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaori","family":"Sadakane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin","family":"Yoshino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kouya","family":"Yamaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiko","family":"Uchiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshikazu","family":"Yoshikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,11,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb16347.x"},{"key":"e_1_2_1_3_1","first-page":"913","article-title":"Endotoxin and lung injury","volume":"133","author":"Brigham KL","year":"1986","journal-title":"Am. 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