{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:33:05Z","timestamp":1790904785417,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fluorescent cellular biomarkers play a prominent role in biosciences. Most of the available biomarkers have some drawbacks due to either physical and optical or cytotoxic properties. In view of this, we investigated the potential of green fluorescent nanodiamonds as biomarkers in living cells. Nanodiamonds were functionalized by attaching antibodies that target intracellular structures such as actin filaments and mitochondria. Then, the nanodiamond conjugates were transfected into HeLa cells. Transfections were mediated by 4<jats:sup>th<\/jats:sup>\u2010generation dendrimers, cationic liposomes and protamine sulfate. Using fluorescence microscopy, we confirmed successful transfections of the nanodiamonds into HeLa cells. Nanodiamond fluorescence could be easily differentiated from cellular autofluorescence. Furthermore, nanodiamonds could be targeted selectively to intracellular structures. Therefore, nanodiamonds are a promising tool for intracellular assays. (\u00a9 2009 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)<\/jats:p>","DOI":"10.1002\/jbio.200910002","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:35:22Z","timestamp":1244212522000},"page":"596-606","source":"Crossref","is-referenced-by-count":93,"title":["Selective targeting of green fluorescent nanodiamond conjugates to mitochondria in HeLa cells"],"prefix":"10.1002","volume":"2","author":[{"given":"Msaukiranji","family":"Mkandawire","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Pohl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Gubarevich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Lapina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietmar","family":"Appelhans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"R\u00f6del","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Pompe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Schreiber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Opitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,9,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124618"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)01714-X"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1717"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605409104"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/smll.200600218"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/smll.200500204"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0567081"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/la0495736"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac048971a"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diamond.2007.07.020"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp066387v"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2008.99"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevB.53.11360"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevB.46.13157"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1134\/1.1711440"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-9635(99)00071-0"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.1777803"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","unstructured":"T. M.GubarevichandD. N.Gamanovich Facilities for ultradisperse diamond production. In Synthesis Properties and Applications of Ultrananocrystalline Diamond NATO Science Series II. Mathematics Physics and Chemistry (D. M. Gruen O. A. Shenderova and A. Y. Vul eds.). 311 (Springer Netherlands 2005).","DOI":"10.1007\/1-4020-3322-2_25"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-2614(01)01362-8"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200800342"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diamond.2007.05.010"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlumin.2007.02.044"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01020-07"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200700987"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc0501451"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300987"},{"key":"e_1_2_1_28_2","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1128\/jb.152.2.765-772.1982","volume":"152","author":"Goscin L. P.","year":"1982","journal-title":"J. Bacteriol."},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608491104"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-2322fje"},{"key":"e_1_2_1_31_2","doi-asserted-by":"crossref","unstructured":"U.Boas J. B.Christennsen andP. M. H.Heegard Dendrimers in Medicine and Biotechnology. (The Royal Society of Chemistry Cambridge 2006).","DOI":"10.1039\/9781847552679-00130"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-4402"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr970069y"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1080\/10611860600635665"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200700051"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1021\/bm049704h"},{"key":"e_1_2_1_37_2","doi-asserted-by":"crossref","unstructured":"T.Dutta M.Garg N.K.andJain Vaccine.26 3389(2008).","DOI":"10.1016\/j.vaccine.2008.04.058"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbio.200910002","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.200910002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T21:26:15Z","timestamp":1699910775000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.200910002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,23]]},"references-count":36,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2009,10]]}},"alternative-id":["10.1002\/jbio.200910002"],"URL":"https:\/\/doi.org\/10.1002\/jbio.200910002","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,23]]}}}