{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T09:05:27Z","timestamp":1789895127692,"version":"4.0.1"},"reference-count":30,"publisher":"Wiley","issue":"10-11","license":[{"start":{"date-parts":[[2010,7,12]],"date-time":"2010-07-12T00:00:00Z","timestamp":1278892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We recently developed an inorganic shell approach for suppressing blinking in nanocrystal quantum dots (NQDs) that has the potential to dramatically improve the utility of these fluorophores for single\u2010NQD tracking of individual molecules in cell biology. Here, we consider in detail the effect of shell thickness and composition on blinking suppression, focusing on the CdSe\/CdS core\/shell system. We also discuss the blinking mechanism as understood through profoundly altered blinking statistics. We clarify the dependence of blinking behavior and photostability on shell thickness, as well as on interrogation times. We show that, while the thickest\u2010shell systems afford the greatest advantages in terms of enhanced optical properties, thinner\u2010shell NQDs may be adequate for certain applications requiring relatively shorter interrogation times. Shell thickness also determines the sensitivity of the NQD optical properties to aqueous\u2010phase transfer, a critical step in rendering NQDs compatible with bioimaging applications. Lastly, we provide a proof\u2010of\u2010concept demonstration of the utility of these unique NQDs for fluorescent particle tracking. (\u00a9 2010 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)<\/jats:p>","DOI":"10.1002\/jbio.201000058","type":"journal-article","created":{"date-parts":[[2010,7,13]],"date-time":"2010-07-13T18:31:29Z","timestamp":1279045889000},"page":"706-717","source":"Crossref","is-referenced-by-count":110,"title":["Effect of shell thickness and composition on blinking suppression and the blinking mechanism in \u2018giant\u2019 CdSe\/CdS nanocrystal quantum dots"],"prefix":"10.1002","volume":"3","author":[{"given":"Javier","family":"Vela","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Htoon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongfen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young\u2010Shin","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yagnaseni","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Goodwin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James H.","family":"Werner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan P.","family":"Wells","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna L.","family":"Casson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer A.","family":"Hollingsworth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,7,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/90228"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja711379k"},{"key":"e_1_2_1_4_2","first-page":"718904","volume":"7189","author":"Hollingsworth J. 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A.Lessard P. M.Goodwin andJ. H.Werner in Ultrasensitive and Single\u2010Molecule Detection Technologies edited by J. Enderlein and Z. K. 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