{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T14:59:37Z","timestamp":1768316377116,"version":"3.49.0"},"reference-count":56,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,6,18]],"date-time":"2012-06-18T00:00:00Z","timestamp":1339977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A preliminary clinical trial using state\u2010of\u2010the\u2010art multiphoton tomography (MPT) and optical coherence tomography (OCT) for three\u2010dimensional (3D) multimodal <jats:italic>in vivo<\/jats:italic> imaging of normal skin, nevi, scars and pathologic skin lesions has been conducted. MPT enabled visualization of sub\u2010cellular details with axial and transverse resolutions of &lt;2 \u03bcm and &lt;0.5 \u03bcm, respectively, from a volume of 0.35 \u00d7 0.35 \u00d7 0.2 mm<jats:sup>3<\/jats:sup> at a frame rate of 0.14 Hz (512 \u00d7 512 pixels). State\u2010of\u2010the\u2010art OCT, operating at a center wavelength of 1300 nm, was capable of acquiring 3D images depicting the layered architecture of skin with axial and transverse resolutions \u223c8 \u03bcm and \u223c20 \u03bcm, respectively, from a volume of 7 \u00d7 3.5 \u00d7 1.5 mm<jats:sup>3<\/jats:sup> at a frame rate of 46 Hz (1024 \u00d7 1024 pixels). This study demonstrates the clinical diagnostic potential of MPT\/OCT for pre\u2010screening relatively large areas of skin using 3D OCT to identify suspicious regions at microscopic level and subsequently using high resolution MPT to obtain zoomed in, sub\u2010cellular level information of the respective regions (\u00a9 2013 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)<\/jats:p>","DOI":"10.1002\/jbio.201200085","type":"journal-article","created":{"date-parts":[[2012,6,18]],"date-time":"2012-06-18T17:22:18Z","timestamp":1340040138000},"page":"352-362","source":"Crossref","is-referenced-by-count":46,"title":["Three\u2010dimensional multiphoton\/optical coherence tomography for diagnostic applications in dermatology"],"prefix":"10.1002","volume":"6","author":[{"given":"Aneesh","family":"Alex","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessika","family":"Weingast","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Weinigel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"Kellner\u2010H\u00f6fer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romina","family":"Nemecek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Binder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hubert","family":"Pehamberger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karsten","family":"K\u00f6nig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Drexler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,6,18]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1431927699990311"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(97)70072-0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12606215"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.1577349"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2009.06.008"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-011-1152-x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/andp.19314010303"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2321027"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.200710022"},{"issue":"5","key":"e_1_2_1_11_2","first-page":"361","volume":"17","author":"Lin S. J.","year":"2007","journal-title":"Eur. J. Dermatol."},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0832308100"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.439"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/810749"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708425104"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1364\/OE.18.007857"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1957169"},{"key":"e_1_2_1_18_2","unstructured":"W.DrexlerandJ. G.Fujimoto(eds.) Optical Coherence Tomography: Technology and Applications 1st edn. (Springer Berlin 2008) p. 1346."},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.3400665"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2005.07.006"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.2217\/iim.11.62"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.3322704"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1364\/OL.24.000969"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.2193428"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1364\/OL.32.000503"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.2171477"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1364\/OE.19.013089"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.3555180"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1364\/OL.36.004800"},{"key":"e_1_2_1_30_2","unstructured":"B.GrafandS.Boppart IEEE J. Sel. Top. Quantum Electron. (2011) DOI 10.1109\/JSTQE.2011.2166377."},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.200910013"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1085-5629(02)80719-2"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.200910058"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1134\/S1054660X10070030"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1364\/OE.3.000212"},{"issue":"1","key":"e_1_2_1_36_2","first-page":"40","volume":"49","author":"Schwartz R. A.","year":"2007","journal-title":"S. Afr. Fam. Pract."},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2010.10180.x"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.4103\/0974-7753.58557"},{"issue":"4","key":"e_1_2_1_39_2","first-page":"170","volume":"21","author":"Bradford P. T.","year":"2009","journal-title":"Dermatol. Nurs."},{"key":"e_1_2_1_40_2","unstructured":"R.\u2010M.Szeimies A.Hauschild C.Garbe R.Kaufmann andM.Landtaler(eds.) Tumoren der Haut: Grundlagen \u2013 Diagnostik \u2013 Therapie (Thieme Stuttgart 2010)."},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800512"},{"key":"e_1_2_1_42_2","unstructured":"M. J.Koehler T.Vogel P.Elsner K.K\u00f6nig R.B\u00fcckle andM.Kaatz Skin Res. Technol. (2010)."},{"key":"e_1_2_1_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mla.2009.07.003"},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0846.2011.00496.x"},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2006.09.008"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/sca.20230"},{"key":"e_1_2_1_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sder.2009.06.007"},{"key":"e_1_2_1_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5701139"},{"key":"e_1_2_1_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2006.11.012"},{"key":"e_1_2_1_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2007.05.029"},{"key":"e_1_2_1_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201100138"},{"key":"e_1_2_1_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2011.01279.x"},{"key":"e_1_2_1_53_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.3520132"},{"key":"e_1_2_1_54_2","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.2.002202"},{"key":"e_1_2_1_55_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.1695564"},{"key":"e_1_2_1_56_2","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.21082"},{"key":"e_1_2_1_57_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201200003"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbio.201200085","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.201200085","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T03:16:36Z","timestamp":1700622996000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.201200085"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,18]]},"references-count":56,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1002\/jbio.201200085"],"URL":"https:\/\/doi.org\/10.1002\/jbio.201200085","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,18]]}}}