{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:58:26Z","timestamp":1777478306891,"version":"3.51.4"},"reference-count":49,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Quantification of erythrocyte zinc protoporphyrin IX (ZnPP) and protoporphyrin IX (PPIX), individually or jointly, is useful for the diagnostic evaluation of iron deficiency, iron\u2010restricted erythropoiesis, lead exposure, and porphyrias. A method for simultaneous quantification of ZnPP and PPIX in unwashed blood samples is described, using dual\u2010wavelength excitation to effectively eliminate background fluorescence from other blood constituents. In blood samples from 35 subjects, the results of the dual\u2010wavelength excitation method and a reference high performance liquid chromatography (HPLC) assay were closely correlated both for ZnPP (<jats:italic>r<jats:sub>s<\/jats:sub><\/jats:italic> = 0.943, <jats:italic>p<\/jats:italic> &lt; 0.0001; range 37\u2013689 \u03bcmol ZnPP\/mol heme, 84\u20131238 nmol\/L) and for PPIX (<jats:italic>r<jats:sub>s<\/jats:sub><\/jats:italic> = 0.959, <jats:italic>p<\/jats:italic> &lt; 0.0001; range 42\u20134212 \u03bcmol PPIX\/mol heme, 93\u20135394 nmol\/L). In addition, for ZnPP, the proposed method is compared with conventional single\u2010wavelength excitation and with commercial front\u2010face fluorimetry of washed erythrocytes and whole blood. We hypothesize that dual\u2010wavelength excitation fluorimetry will provide a new approach to the suppression of background fluorescence in blood and tissue measurements of ZnPP and PPIX. (\u00a9 2014 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)<\/jats:p>","DOI":"10.1002\/jbio.201200228","type":"journal-article","created":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T19:27:36Z","timestamp":1362166056000},"page":"514-524","source":"Crossref","is-referenced-by-count":16,"title":["Dual\u2010wavelength excitation for fluorescence\u2010based quantification of zinc protoporphyrin IX and protoporphyrin IX in whole blood"],"prefix":"10.1002","volume":"7","author":[{"given":"Georg","family":"Hennig","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Gruber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Vogeser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herbert","family":"Stepp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan","family":"Dittmar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald","family":"Sroka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary M.","family":"Brittenham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,3]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2012.04.009"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.1093\/clinchem\/45.12.2060","volume":"45","author":"Labbe R. F.","year":"1999","journal-title":"Clin. Chem."},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(89)90191-5"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.2486\/indhealth.38.372"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61235-5"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.3945\/jn.110.130609"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-05-286260"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(00)00149-1"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1182\/blood.V81.5.1200.1200","volume":"81","author":"Hastka J.","year":"1993","journal-title":"Blood"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-007-0271-0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2008.08.003"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-4-19"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0738-081X(97)00207-1"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61925-5"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.186.4167.936"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00716375"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1615\/JEnvironPatholToxicolOncol.v29.i2.70"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2011.1.525"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.13619"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1366\/000370210791114248"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1093\/clinchem\/29.8.1467","volume":"29","author":"Kaul B.","year":"1983","journal-title":"Clin. Chem."},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1130010407"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1093\/clinchem\/27.2.220","volume":"27","author":"Hart D.","year":"1981","journal-title":"Clin. Chem."},{"key":"e_1_2_1_25_2","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1093\/clinchem\/32.12.2137","volume":"32","author":"Bailey G. G.","year":"1986","journal-title":"Clin. Chem."},{"key":"e_1_2_1_26_2","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1093\/clinchem\/40.7.1239","volume":"40","author":"Sato H.","year":"1994","journal-title":"Clin. Chem."},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2005.082"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1080\/10715760500522630"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1366\/000370208786401536"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2011.11.057"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/23.2.270"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00430a063"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1177\/15648265070284S414"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1177\/15648265070284S410"},{"key":"e_1_2_1_35_2","first-page":"710","volume":"91","author":"Buhrmann E.","year":"1978","journal-title":"J. Lab. Clin. Med."},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.5271\/sjweh.2542"},{"key":"e_1_2_1_37_2","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1093\/clinchem\/38.11.2184","volume":"38","author":"Hastka J.","year":"1992","journal-title":"Clin. Chem."},{"key":"e_1_2_1_38_2","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1093\/clinchem\/45.1.146","volume":"45","author":"Labbe R. F.","year":"1999","journal-title":"Clin. Chem."},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(85)80076-X"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/27.1.153"},{"key":"e_1_2_1_41_2","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1093\/clinchem\/35.10.2059","volume":"35","author":"Parsons P. J.","year":"1989","journal-title":"Clin. Chem."},{"key":"e_1_2_1_42_2","unstructured":"S. A.Prahl http:\/\/omlc.ogi.edu\/spectra\/hemoglobin\/index.html (1999) retrieved 27.04.2010."},{"key":"e_1_2_1_43_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110664"},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.3006066"},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11307-011-0501-7"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00704a022"},{"key":"e_1_2_1_47_2","unstructured":"ImmundiagnostikAG http:\/\/www.immundiagnostik.com\/fileadmin\/pdf\/Zink\u2010Protoporphyrin_KC2700.pdf (2011) retrieved 20.08.2012."},{"key":"e_1_2_1_48_2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2001.90.2.511"},{"key":"e_1_2_1_49_2","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1182\/blood.V62.4.846.846","volume":"62","author":"Sandberg S.","year":"1983","journal-title":"Blood"},{"key":"e_1_2_1_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990130403"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbio.201200228","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.201200228","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T23:42:36Z","timestamp":1700610156000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.201200228"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3]]},"references-count":49,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["10.1002\/jbio.201200228"],"URL":"https:\/\/doi.org\/10.1002\/jbio.201200228","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3]]}}}