{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:32:06Z","timestamp":1761129126990},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,1,31]],"date-time":"2014-01-31T00:00:00Z","timestamp":1391126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Intravital fluorescence microscopy (IVM) is a predestined tool for investigating the fate of leukocytes during the process of leukocyte recruitment. In the present study, the commonly used dye for this purpose, rhodamine 6G, and carboxyfluorescein diacetate succinimidyl ester (CFDA\u2010SE) were compared for leukocytes labelling with respect to suitability for IVM studies. Their potential in labelling different leukocytes subpopulations as well as their fluorescence intensities were assessed by flow cytometry revealing distinct differences between both dyes. These differences had a profound impact on their application for <jats:italic>in vivo<\/jats:italic> imaging of leukocyte\u2010endothelium interactions. In summary, CFDA\u2010SE revealed superior in labelling leukocytes for <jats:italic>in vivo<\/jats:italic> microscopy with respect to image quality. In addition, we could show the efficiency of CFDA\u2010SE also under disease condition in an animal model of sepsis. (\u00a9 2014 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)<\/jats:p>","DOI":"10.1002\/jbio.201300179","type":"journal-article","created":{"date-parts":[[2014,1,31]],"date-time":"2014-01-31T12:56:54Z","timestamp":1391173014000},"page":"369-375","source":"Crossref","is-referenced-by-count":9,"title":["Comparative suitability of CFDA\u2010SE and rhodamine 6G for <i>in vivo<\/i> assessment of leukocyte\u2010endothelium interactions"],"prefix":"10.1002","volume":"7","author":[{"given":"Nayla","family":"Jbeily","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf A.","family":"Claus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katja","family":"Dahlke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ute","family":"Neugebauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Falk A.","family":"Gonnert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,31]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90179-O"},{"key":"e_1_2_1_3_2","first-page":"542","volume":"28","author":"Menger M. 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