{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:37:14Z","timestamp":1790743034096,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,7]],"date-time":"2015-04-07T00:00:00Z","timestamp":1428364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council (NMRC)","doi-asserted-by":"crossref","award":["NMRC\/CIRG\/1331\/2012; BnB13dec037"],"award-info":[{"award-number":["NMRC\/CIRG\/1331\/2012; BnB13dec037"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Research Foundation-Proof-of-Concept (NRF-POC)","award":["NRF2014NRF-POC001-012"],"award-info":[{"award-number":["NRF2014NRF-POC001-012"]}]},{"name":"Academic Research Fund (AcRF)-Tier 2 from the Ministry of Education (MOE)","award":["MOE2014-T2-1-010"],"award-info":[{"award-number":["MOE2014-T2-1-010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:p>Colorectal cancer can be prevented if detected early (e.g., precancerous polyps\u2010adenoma). Endoscopic differential diagnosis of hyperplastic polyps (that have little or no risk of malignant transformation) and adenomas (that have prominent malignant latency) remains an unambiguous clinical challenge. Raman spectroscopy is an optical vibrational technique capable of probing biomolecular changes of tissue associated with neoplastic transformation. This work aims to apply a fiber\u2010optic simultaneous fingerprint (FP) and high wavenumber (HW) Raman spectroscopy technique for real\u2010time <jats:italic>in vivo<\/jats:italic> assessment of adenomatous polyps during clinical colonoscopy. We have developed a fiber\u2010optic Raman endoscopic technique capable of simultaneously acquiring both the FP (i.e., 800\u20131800\u00a0cm<jats:sup>\u20131<\/jats:sup>) and HW (i.e., 2800\u20133600\u00a0cm<jats:sup>\u20131<\/jats:sup>) Raman spectra from colorectal tissue subsurface (&lt;200 \u00b5m) for real\u2010time assessment of colorectal carcinogenesis. In vivo FP\/HW Raman spectra were acquired from 50 patients with 17 colorectal polyps during clinical colonoscopy. Prominent Raman spectral differences (<jats:italic>p<\/jats:italic> &lt; 0.001) were found between hyperplastic (<jats:italic>n<\/jats:italic> = 118 spectra), adenoma (<jats:italic>n<\/jats:italic> = 184 spectra) that could be attributed to changes in inter\u2010 and intra\u2010cellular proteins, lipids, DNA and water structures and conformations. Simultaneous FP\/HW Raman endoscopy provides a diagnostic sensitivity of 90.9% and specificity of 83.3% for differentiating adenoma from hyperplastic polyps, which is superior to either the FP or HW Raman technique alone. This study shows that simultaneous FP\/HW Raman spectroscopy technique has the potential to be a clinically powerful tool for improving early diagnosis of adenomatous polyps <jats:italic>in vivo<\/jats:italic> during colonoscopic examination.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jbio201400141-gra-0001-m.png\"><jats:alt-text>magnified image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p>","DOI":"10.1002\/jbio.201400141","type":"journal-article","created":{"date-parts":[[2015,4,7]],"date-time":"2015-04-07T16:53:11Z","timestamp":1428425591000},"page":"333-342","source":"Crossref","is-referenced-by-count":89,"title":["Simultaneous fingerprint and high\u2010wavenumber fiber\u2010optic Raman spectroscopy enhances real\u2010time <i>in vivo<\/i> diagnosis of adenomatous polyps during colonoscopy"],"prefix":"10.1002","volume":"9","author":[{"given":"Mads Sylvest","family":"Bergholt","sequence":"first","affiliation":[{"name":"Optical Bioimaging Laboratory Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore  9 Engineering Drive 1 Singapore 117576 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kan","family":"Lin","sequence":"additional","affiliation":[{"name":"Optical Bioimaging Laboratory Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore  9 Engineering Drive 1 Singapore 117576 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Optical Bioimaging Laboratory Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore  9 Engineering Drive 1 Singapore 117576 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Optical Bioimaging Laboratory Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore  9 Engineering Drive 1 Singapore 117576 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhi","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Digestive Disease Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University  Xiamen 361004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingwen","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Digestive Disease Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University  Xiamen 361004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010lin","family":"Ren","sequence":"additional","affiliation":[{"name":"Institute of Digestive Disease Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University  Xiamen 361004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khek Yu","family":"Ho","sequence":"additional","affiliation":[{"name":"Department of Medicine Yong Loo Lin School of Medicine, National University of Singapore and National University Health System  Singapore 119260 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Teh","sequence":"additional","affiliation":[{"name":"Department of Pathology Yong Loo Lin School of Medicine, National University of Singapore and National University Health System  Singapore 119074 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supriya","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Pathology Yong Loo Lin School of Medicine, National University of Singapore and National University Health System  Singapore 119074 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Pathology Yong Loo Lin School of Medicine, National University of Singapore and National University Health System  Singapore 119074 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khay Guan","family":"Yeoh","sequence":"additional","affiliation":[{"name":"Department of Medicine Yong Loo Lin School of Medicine, National University of Singapore and National University Health System  Singapore 119260 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwei","family":"Huang","sequence":"additional","affiliation":[{"name":"Optical Bioimaging Laboratory Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore  9 Engineering Drive 1 Singapore 117576 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,4,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20107"},{"key":"e_1_2_6_3_1","first-page":"8","volume":"2012","author":"Quintero E.","year":"2012","journal-title":"Gastroenterolog. Res. Practice"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1067\/mge.2003.105"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2010.83"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.12.067"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/121501c0"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11500"},{"key":"e_1_2_6_9_1","first-page":"653","volume":"32","author":"Widjaja E.","year":"2008","journal-title":"Int. J. Oncol"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.38.002321"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.34.000758"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.882"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac300394f"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.17.8.081418"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.18.3.030502"},{"key":"e_1_2_6_16_1","first-page":"249","volume":"22","author":"Carletta J.","year":"1996","journal-title":"Comput. Linguist"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24935"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.6913"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2013.11.002"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0an00336k"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.143.1.7063747"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0101306"},{"key":"e_1_2_6_23_1","doi-asserted-by":"crossref","first-page":"837","DOI":"10.2307\/2531595","volume":"4","author":"DeLong E. R.","year":"1988","journal-title":"Biometrics"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.103.038604"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2007.125286"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206762"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.21.11312"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbio.201400141","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.201400141","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T22:08:13Z","timestamp":1696457293000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.201400141"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,7]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,4]]}},"alternative-id":["10.1002\/jbio.201400141"],"URL":"https:\/\/doi.org\/10.1002\/jbio.201400141","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,7]]}}}