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Pathological alterations of microvilli structure affect the tear film stability and, conversely, dysfunctions of tear film composition can lead to a suffering epithelium (dry\u2010eye syndrome). In this work we propose the use of micro\u2010Raman analysis to reveal conjunctival microvilli abnormalities. Samples were obtained by impression cytology from patients by different stage of dry\u2010eye syndrome. Our experimental outcomes demonstrate that Raman analysis, combined with the use of Principal Component Analysis, is able to detect different stages of microvilli reduction. Globally, these results hold promise for the use of Raman analysis for an objective, effective, non\u2010invasive and potentially also <jats:italic>in\u2010vivo<\/jats:italic> analysis of the conjunctiva in all the cases of microvilli\u2010related ocular pathologies.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jbio201500278-gra-0001-m.png\"><jats:alt-text>magnified image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p>","DOI":"10.1002\/jbio.201500278","type":"journal-article","created":{"date-parts":[[2016,2,15]],"date-time":"2016-02-15T09:42:50Z","timestamp":1455529370000},"page":"551-559","source":"Crossref","is-referenced-by-count":8,"title":["Assessment of conjunctival microvilli abnormality by micro\u2010Raman analysis \u2013 by G. Rusciano et al"],"prefix":"10.1002","volume":"9","author":[{"given":"Giulia","family":"Rusciano","sequence":"first","affiliation":[{"name":"Department of Physics E. Pancini University of Naples Federico II  via Cintia 80126\u2010I Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianluigi","family":"Zito","sequence":"additional","affiliation":[{"name":"Department of Physics E. Pancini University of Naples Federico II  via Cintia 80126\u2010I Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Pesce","sequence":"additional","affiliation":[{"name":"Department of Physics E. 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