{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:10:21Z","timestamp":1789769421833,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T00:00:00Z","timestamp":1464652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Special Fund for Agro-scientific Research in the Public Interest of the Ministry of agriculture","award":["201203040"],"award-info":[{"award-number":["201203040"]}]},{"name":"National Science & Technology Pillar Program of \u201812th Five-Year Plan'","award":["2014BAD13B05"],"award-info":[{"award-number":["2014BAD13B05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:p>Novel direct and indirect competitive fluorescence\u2010linked immunosorbent assays (<jats:italic>c<\/jats:italic>FLISA and<jats:italic> ic<\/jats:italic>FLISA) for detection of ochratoxin A (OTA) were described using CdTe quantum dots (QDs) as fluorescent label. CdTe QDs were successfully synthesized, which had an emission wavelength of 615\u00a0nm. The high purity monoclonal antibody against OTA was prepared through cell thawing and the octylic acid\u2010ammonium sulfate method. The OTA MAbs were successfully coupled with CdTe QDs, and which also retained the original biological activity. The 50% inhibition values (IC<jats:sub>50<\/jats:sub>) of the <jats:italic>c<\/jats:italic>FLISA and <jats:italic>ic<\/jats:italic>FLISA were 0.630\u00a0ng\/mL, 0.234\u00a0ng\/mL, the limits of detection (LOD) were 7.06\u00a0\u00d7\u00a010<jats:sup>\u20133<\/jats:sup> and 4.15\u00a0\u00d7\u00a010<jats:sup>\u20133<\/jats:sup>\u00a0ng\/mL, and detection ranges were 7.06\u00a0\u00d7\u00a010<jats:sup>\u20133<\/jats:sup> \u2212 18.34\u00a0ng\/mL and 4.15\u00a0\u00d7\u00a010<jats:sup>\u20133<\/jats:sup> \u2212 4.88\u00a0ng\/mL, in\u2010order. The recoveries were 96.0\u2013104.7% along with coefficients of variation (CVs) below 10%. The FLISA provided novel method for determination of OTA and the potential of MAb\u2013CdTe QDs for the establishment of fluorescent immunochromatographic test strip.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jbio201600005-gra-0001-m.png\"><jats:alt-text>magnified image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p>","DOI":"10.1002\/jbio.201600005","type":"journal-article","created":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T20:14:58Z","timestamp":1464725698000},"page":"657-663","source":"Crossref","is-referenced-by-count":27,"title":["Novel fluoroimmunoassays for detecting ochratoxin A using CdTe quantum dots"],"prefix":"10.1002","volume":"10","author":[{"given":"Jingjing","family":"Yao","sequence":"first","affiliation":[{"name":"Henan Academy of Agriculture Science\/Key laboratory of animal Immunology Ministry of Agriculture\/Henan key laboratory of animal Immunology  Zhengzhou 450002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangxu","family":"Xing","sequence":"additional","affiliation":[{"name":"Henan Academy of Agriculture Science\/Key laboratory of animal Immunology Ministry of Agriculture\/Henan key laboratory of animal Immunology  Zhengzhou 450002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junling","family":"Han","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xinxiang Medical University  Xinxiang 453100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaning","family":"Sun","sequence":"additional","affiliation":[{"name":"Henan Academy of Agriculture Science\/Key laboratory of animal Immunology Ministry of Agriculture\/Henan key laboratory of animal Immunology  Zhengzhou 450002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Henan Academy of Agriculture Science\/Key laboratory of animal Immunology Ministry of Agriculture\/Henan key laboratory of animal Immunology  Zhengzhou 450002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiguang","family":"Deng","sequence":"additional","affiliation":[{"name":"Henan Academy of Agriculture Science\/Key laboratory of animal Immunology Ministry of Agriculture\/Henan key laboratory of animal Immunology  Zhengzhou 450002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofei","family":"Hu","sequence":"additional","affiliation":[{"name":"Henan Academy of Agriculture Science\/Key laboratory of animal Immunology Ministry of Agriculture\/Henan key laboratory of animal Immunology  Zhengzhou 450002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaiping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Henan Academy of Agriculture Science\/Key laboratory of animal Immunology Ministry of Agriculture\/Henan key laboratory of animal Immunology  Zhengzhou 450002 China"},{"name":"Henan Agricultural University  Zhengzhou 450002 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"2650","DOI":"10.1128\/aem.60.7.2650-2652.1994","volume":"60","author":"Abarca M. 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