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Many studies have been performed to understand lymphatic function under normal conditions and during disease. Nevertheless, a further improvement in quantification of lymphatic behavior is needed. Here, we present advanced bright\u2010field microscopy for in vivo imaging of lymph vessels (LVs) and automated quantification of lymphatic function at a temporal resolution of 2 milliseconds. Full frame videos were compressed and recorded continuously at up to 540 frames per second. A new edge detection algorithm was used to monitor vessel diameter changes across multiple cross sections, while individual cells in the LVs were tracked to estimate flow velocity. The system performance initially was verified in vitro using 6\u2010 and 10\u2010\u03bcm microspheres as cell phantoms on slides and in 90\u2010\u03bcm diameter tubes at flow velocities up to 4 cm\/second. Using an in vivo rat model, we explored the mechanisms of lymphedema after surgical lymphadenectomy of the mesentery. The system revealed reductions of mesenteric LV contraction and flow rate. Thus, the described imaging system may be applicable to the study of lymphatic behavior during therapeutic and surgical interventions, and potentially during lymphatic system diseases.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio201700126-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.201700126","type":"journal-article","created":{"date-parts":[[2017,12,12]],"date-time":"2017-12-12T19:43:23Z","timestamp":1513107803000},"source":"Crossref","is-referenced-by-count":14,"title":["High\u2010speed microscopy for in vivo monitoring of lymph dynamics"],"prefix":"10.1002","volume":"11","author":[{"given":"Mustafa","family":"Sarimollaoglu","sequence":"first","affiliation":[{"name":"Arkansas Nanomedicine Center University of Arkansas for Medical Sciences  Little Rock Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda J.","family":"Stolarz","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology University of Arkansas for Medical Sciences  Little Rock Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry A.","family":"Nedosekin","sequence":"additional","affiliation":[{"name":"Arkansas Nanomedicine Center University of Arkansas for Medical Sciences  Little Rock Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brittney R.","family":"Garner","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology University of Arkansas for Medical Sciences  Little Rock Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry W.","family":"Fletcher","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology University of Arkansas for Medical Sciences  Little Rock Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina I.","family":"Galanzha","sequence":"additional","affiliation":[{"name":"Arkansas Nanomedicine Center University of Arkansas for Medical Sciences  Little Rock Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy J.","family":"Rusch","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology University of Arkansas for Medical Sciences  Little Rock Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir P.","family":"Zharov","sequence":"additional","affiliation":[{"name":"Arkansas Nanomedicine Center University of Arkansas for Medical Sciences  Little Rock Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18010171"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i2.192"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20587"},{"key":"e_1_2_7_5_1","first-page":"875","volume-title":"Handbook of Optical Biomedical Diagnostics","author":"Galanzha E. 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