{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:39:22Z","timestamp":1792161562629,"version":"4.4.0"},"reference-count":56,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,1,29]],"date-time":"2018-01-29T00:00:00Z","timestamp":1517184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Health & Welfare, Republic of Korea","award":["HI16C-0272-010016"],"award-info":[{"award-number":["HI16C-0272-010016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:p>Recent zoonotic outbreaks, such as Zika, Middle East respiratory syndrome and Ebola, have highlighted the need for rapid and accurate diagnostic assays that can be used to aid pathogen control. Q fever is a zoonotic disease caused by the transmission of <jats:italic>Coxiella burnetii<\/jats:italic> that can cause serious illness in humans through aerosols and is considered a potential bioterrorism agent. However, the existing assays are not suitable for the detection of this pathogen due to its low levels in real samples. We here describe a rapid bio\u2010optical sensor for the accurate detection of Q fever and validate its clinical utility. By combining a bio\u2010optical sensor, that transduces the presence of the target DNA based on binding\u2010induced changes in the refractive index on the waveguide surface in a label\u2010free and real\u2010time manner, with isothermal DNA amplification, this new diagnostic tool offers a rapid (&lt;20\u2009min), 1\u2010step DNA amplification\/detection method. We confirmed the clinical sensitivity (&gt;90%) of the bio\u2010optical sensor by detecting <jats:italic>C. burnetii<\/jats:italic> in 11 formalin\u2010fixed, paraffin\u2010embedded liver biopsy samples from acute Q fever hepatitis patients and in 16 blood plasma samples from patients in which Q fever is the cause of fever of unknown origin.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio201700167-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.201700167","type":"journal-article","created":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T14:22:39Z","timestamp":1507645359000},"source":"Crossref","is-referenced-by-count":12,"title":["A rapid bio\u2010optical sensor for diagnosing Q fever in clinical specimens"],"prefix":"10.1002","volume":"11","author":[{"given":"Bonhan","family":"Koo","sequence":"first","affiliation":[{"name":"Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine and the Biomedical Engineering Research Center Asan Institute of Life Sciences 88 Olympicro\u201043gil, Songpa\u2010gu  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Choong Eun","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine and the Biomedical Engineering Research Center Asan Institute of Life Sciences 88 Olympicro\u201043gil, Songpa\u2010gu  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se Yoon","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine Soonchunhyang University College of Medicine 59 Daesangwan\u2010ro, Yongsan\u2010gu  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Yoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Technology Education Chungnam National University  Daejeon 34134 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeonghun","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, School of Medicine Korea University 145 Anam\u2010ro, Seongbuk\u2010gu  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young\u2010Rock","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Asan Medical Center University of Ulsan College of Medicine 88 Olympicro\u201043gil, Songpa\u2010gu  Seoul Republic of Korea"},{"name":"Division of Infectious Disease, Department of Internal Medicine, Gil Medical Center Gachon University College of Medicine, 21 Namdong\u2010daero 774 beon\u2010gil  Namdong\u2010gu, Incheon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Mi","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Asan Medical Center University of Ulsan College of Medicine 88 Olympicro\u201043gil, Songpa\u2010gu  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Yeun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Asan Medical Center University of Ulsan College of Medicine 88 Olympicro\u201043gil, Songpa\u2010gu  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung\u2010Han","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Asan Medical Center University of Ulsan College of Medicine 88 Olympicro\u201043gil, Songpa\u2010gu  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine and the Biomedical Engineering Research Center Asan Institute of Life Sciences 88 Olympicro\u201043gil, Songpa\u2010gu  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0903.020208"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1247566"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature16041"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw239"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.04.059"},{"key":"e_1_2_7_7_1","first-page":"615","volume":"98","author":"Rolain J. 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