{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T14:55:33Z","timestamp":1774882533528,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T00:00:00Z","timestamp":1516233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2011\/14013\u20101"],"award-info":[{"award-number":["2011\/14013\u20101"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2012\/02739\u20100"],"award-info":[{"award-number":["2012\/02739\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2012\/20845\u20102"],"award-info":[{"award-number":["2012\/20845\u20102"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE025726"],"award-info":[{"award-number":["DE025726"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:p>Lipid droplet accumulation has been related to salivary gland hypofunction in diabetes. In this study, the effect of laser irradiation on the parotid glands (PGs) of diabetic rats was analyzed with regard to its effect on lipid droplet accumulation, intracellular calcium concentration and calmodulin expression. The animals were distributed into 6 groups: D0, D5, D20 and C0, C5, C20, for diabetic (D) and control animals (C), respectively. Twenty\u2010nine days following diabetes induction, PGs of groups D5 and C5; D20 and C20 were irradiated with 5 and 20\u2009J\/cm<jats:sup>2<\/jats:sup> of a red diode laser at 100\u2009mW, respectively. After 24\u2009hours, PGs were removed for histological, biochemical, and western blotting analysis. The diabetic animals showed lipid droplet accumulation, which was decreased after irradiation. Ultrastructurally, the droplets were nonmembrane bound and appeared irregularly located in the cytoplasm. Moreover, diabetic animals showed an increased intracellular calcium concentration. In contrast, after laser irradiation a progressive decrease in the concentration of this ion was observed, which would be in agreement with the results found in the increased expression of calmodulin in D20. These data are promising for using laser to decrease lipid droplet accumulation in PGs, however, more studies are necessary to better understand its mechanisms. Micrographs showing decreased lipid accumulation after laser irradiation in light micrographs (LM), and morphology of lipid droplet in transmission electron microscopic (TEM). LM: (A) PGs from nondiabetic rats that did not receive Laser irradiation (LI), (B) PGs from nondiabetic rats that received a dose of 20\u2009J\/cm<jats:sup>2<\/jats:sup>, (C) lipid accumulation (arrows) in the secretory cells from diabetic rats that did not receive irradiation, (D) reduction of lipid accumulation in the secretory cells from diabetic rats that received a dose of 20\u2009J\/cm<jats:sup>2<\/jats:sup> and TEM: (E) scale bar\u2009=\u20095 \u03bcm, (F) scale bar\u2009=\u20091 \u03bcm, and (G) scale bar\u2009=\u20090.5 \u03bcm.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio201700179-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.201700179","type":"journal-article","created":{"date-parts":[[2017,11,7]],"date-time":"2017-11-07T13:46:43Z","timestamp":1510062403000},"source":"Crossref","is-referenced-by-count":7,"title":["Low\u2010power laser irradiation decreases lipid droplet accumulation in the parotid glands of diabetic rats"],"prefix":"10.1002","volume":"11","author":[{"given":"Juliana R.","family":"de Castro","sequence":"first","affiliation":[{"name":"Laboratory of Oral Biology, Department of Biomaterials and Oral Biology, School of Dentistry University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugen M. N.","family":"de Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Biology, Department of Biomaterials and Oral Biology, School of Dentistry University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun\u2010Jong","family":"Park","sequence":"additional","affiliation":[{"name":"Oral Maxillofacial Diagnostic Sciences, College of Dentistry University of Florida  Gainesville Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luana","family":"de Campos","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Biology, Department of Biomaterials and Oral Biology, School of Dentistry University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Department of Oral Medicine Brazilian Institute for Cancer Control  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seunghee","family":"Cha","sequence":"additional","affiliation":[{"name":"Oral Maxillofacial Diagnostic Sciences, College of Dentistry University of Florida  Gainesville Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor E.","family":"Arana\u2010Chavez","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Biology, Department of Biomaterials and Oral Biology, School of Dentistry University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Nicolau","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Biology, Department of Biomaterials and Oral Biology, School of Dentistry University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4873-1393","authenticated-orcid":false,"given":"Alyne","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Biology, Department of Biomaterials and Oral Biology, School of Dentistry University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-012-1173-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508400604"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.1544"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2334\/josnusd.47.111"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-008-0619-2"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.1555"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2008.2452"},{"key":"e_1_2_7_9_1","first-page":"429","volume":"51","author":"Hand A. R.","year":"1984","journal-title":"Lab. Invest."},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-008-8233-5"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201600175"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169443"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529-8027.2007.00114.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2010.2789"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2006.24.33"},{"key":"e_1_2_7_16_1","volume-title":"Lasers in Dentistry: Guide for Clinical Practice","year":"2015"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1754-4505.2009.00078.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2009.2486"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2009.2495"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-77095-1"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2613.2009.00662.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(95)02102-7"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(86)90021-X"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-005-0367-5"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-200209010-00030"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.22625"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.22007"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cps.2005.09.005"},{"key":"e_1_2_7_29_1","first-page":"48","volume":"16","author":"Schilling L.","year":"2017","journal-title":"J. Drugs Dermatol."},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2015.05.017"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/39.5.815"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2015.02.003"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(89)90325-1"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(02)00049-3"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(79)90240-7"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(97)00050-2"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345970760030601"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbio.201700179","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.201700179","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T10:19:47Z","timestamp":1694945987000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.201700179"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,18]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1002\/jbio.201700179"],"URL":"https:\/\/doi.org\/10.1002\/jbio.201700179","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,18]]},"article-number":"e201700179"}}