{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T02:55:07Z","timestamp":1778986507194,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,5,17]],"date-time":"2018-05-17T00:00:00Z","timestamp":1526515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008316","name":"Baden-W\u00fcrttemberg Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008316","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004079","name":"Konrad-Adenauer-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004079","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:p>A laser's high degree of coherence leads to interferences, which\u2014in the absence of precautions\u2014can cause severe image distortions such as fringes and speckles and which thereby strongly hamper a meaningful interpretation of hyperspectral images in laser\u2010based widefield microspectroscopy. While images and spectra of homogenous samples may already suffer from interferences, any structured object such as a tissue thin section will add to these distortions due to wavelength\u2010 and, in particular, sample\u2010dependent phase shifts (structure sizes, absorption coefficients, refractive indices). This effect is devastating for the universal applicability of laser\u2010based microspectroscopy especially in the mid\u2010infrared (MIR), where cell sizes are of the same dimension as the wavelength of the illumination source. Here, we show that the impact of interferences is strongly mitigated by reducing the time\u2010averaged spatiotemporal coherence properties of the illumination using a moving plus a stationary scatterer. In this case, the illumination path provides a pseudothermal radiation source and spatially resolved spectra can be obtained at the quality of the reference method, that is, Fourier\u2010transform infrared microspectroscopy, without compromising spectral or spatial resolution.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio201800015-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.201800015","type":"journal-article","created":{"date-parts":[[2018,3,24]],"date-time":"2018-03-24T03:51:44Z","timestamp":1521863504000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["On the role of interference in laser\u2010based mid\u2010infrared widefield microspectroscopy"],"prefix":"10.1002","volume":"11","author":[{"given":"Arthur","family":"Sch\u00f6nhals","sequence":"first","affiliation":[{"name":"Kirchhoff Institute for Physics Heidelberg University  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niels","family":"Kr\u00f6ger\u2010Lui","sequence":"additional","affiliation":[{"name":"Kirchhoff Institute for Physics Heidelberg University  Heidelberg Germany"},{"name":"IRM<sup>2<\/sup> GmbH  Eppelheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annemarie","family":"Pucci","sequence":"additional","affiliation":[{"name":"Kirchhoff Institute for Physics Heidelberg University  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Petrich","sequence":"additional","affiliation":[{"name":"Kirchhoff Institute for Physics Heidelberg University  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,5,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470283172"},{"key":"e_1_2_6_3_1","volume-title":"Introduction to Modern Vibrational Spectroscopy","author":"Diem M.","year":"1993"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.264.5158.553"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.16.001836"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.OE.52.6.061302"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac302513f"},{"key":"e_1_2_6_8_1","series-title":"Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry","volume-title":"Proc. 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